NCTC clone 2472 (ATCC® CCL-11)

Organism: Mus musculus, mouse  /  Tissue: subcutaneous connective tissue; areolar and adipose  /  Disease: sarcoma

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Organism Mus musculus, mouse
Tissue subcutaneous connective tissue; areolar and adipose
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease sarcoma
Age 82 days
Gender male
Strain C3H/HeN
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 52; range = 47 to 173, An extra long telocentric demonstrable in 100/100 cells. No metacentrics were observed. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.
Derivation

NCTC clone 2472 was derived in May, 1956, by K.K. Sanford, et al. from NCTC strain 1742 (Subline VII) of the parent NCTC clone 1328.

The parent line was derived by G.D. Likely et al. in November, 1950, from a culture of subcutaneous areolar and adipose connective tissue taken from a normal 82-day-old male C3H/HeN mouse.

Clinical Data
male
Tumorigenic yes, In non-treated C3H/HeN mice, sarcomas developed within 12 days in 20/20 mice injected intramuscularly with 3.2 X 10(6) cells. In irradiated mice (425 r whole body), tumors developed within 12 days in 18/18 mice injected with 3.2 X 10(6) cells.
Effects
Yes, In irradiated mice (425 r whole body)
Yes, in untreated C3H/HeN mice
Virus Susceptibility Vesicular stomatitis, Orsay (Indiana)
Vesicular stomatitis, Glasgow (Indiana)
Virus Resistance
poliovirus 3
Comments

While cultures of NCTC strain 1742 produced sarcomas in 97% of the mice injected, cultures of NCTC clone 2472 under similar conditions produced sarcomas in 65% of the mice injected.

NCTC clone 2472 has been compared with related clones, such as NCTC clone 2555 (ATCC CCL-12), and certain other cell lines with respect to neoplastic and physiologic properties, nutitional needs, karyology, enzyme activities, and rates of glycolysis.

NCTC clone 2472 cells have been cultured since origin in an antibiotic-free mixture of medium NCTC 109 (135) with 10% horse serum.

Plating efficiency is approximately 10% in the recommended culture medium.

Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium NCTC 135 medium, 90%; horse serum, 10%
Subculturing

Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended

Medium Renewal: 3 times per week

Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Name of Depositor KK Sanford
Year of Origin 1956
References

Sanford KK, et al. Clonal analysis of variant cell lines transformed to malignant cells in tissue culture. J. Natl. Cancer Inst. 23: 1035-1059, 1959. PubMed: 14441477

Likely GD, et al. Further studies on the proliferation in vitro of single isolated tissue cells. J. Natl. Cancer Inst. 13: 177-184, 1952. PubMed: 14946507

Sanford KK, et al. The development of variations in transplantability and morphology within a clone of mouse fibroblasts transformed to sarcoma-producing cells in vitro. J. Natl. Cancer Inst. 15: 215-237, 1954. PubMed: 13233880

Sanford KK. Clonal studies on normal cells and on their neoplastic transformation in vitro. Cancer Res. 18: 747-752, 1958. PubMed: 13573337

Sanford KK, et al. Studies on the difference in sarcoma-producing capacity of two lines of mouse cells derived in vitro from one cell. J. Natl. Cancer Inst. 20: 121-145, 1958. PubMed: 13502776

Scott DB, et al. Analysis of enzymatic activities of clones derived from variant cell lines transformed to malignant cells in tissue culture. J. Natl. Cancer Inst. 25: 1365-1379, 1960. PubMed: 13749340

Dupree, L. T. , et al. Influence of serum protein on determination of nutritional requirements of cells in culture, Exp. Cell Res., 28, 381 (1962)

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These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Sanford KK, et al. Clonal analysis of variant cell lines transformed to malignant cells in tissue culture. J. Natl. Cancer Inst. 23: 1035-1059, 1959. PubMed: 14441477

Likely GD, et al. Further studies on the proliferation in vitro of single isolated tissue cells. J. Natl. Cancer Inst. 13: 177-184, 1952. PubMed: 14946507

Sanford KK, et al. The development of variations in transplantability and morphology within a clone of mouse fibroblasts transformed to sarcoma-producing cells in vitro. J. Natl. Cancer Inst. 15: 215-237, 1954. PubMed: 13233880

Sanford KK. Clonal studies on normal cells and on their neoplastic transformation in vitro. Cancer Res. 18: 747-752, 1958. PubMed: 13573337

Sanford KK, et al. Studies on the difference in sarcoma-producing capacity of two lines of mouse cells derived in vitro from one cell. J. Natl. Cancer Inst. 20: 121-145, 1958. PubMed: 13502776

Scott DB, et al. Analysis of enzymatic activities of clones derived from variant cell lines transformed to malignant cells in tissue culture. J. Natl. Cancer Inst. 25: 1365-1379, 1960. PubMed: 13749340

Dupree, L. T. , et al. Influence of serum protein on determination of nutritional requirements of cells in culture, Exp. Cell Res., 28, 381 (1962)