L-M(TK-) [LM(tk-), LMTK-] (ATCC® CCL-1.3)

Organism: Mus musculus  /  Tissue: subcutaneous connective tissue; areolar and adipose  / 

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Organism Mus musculus
Tissue subcutaneous connective tissue; areolar and adipose
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 100 days
Gender male
Strain C3H/An
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 45; range = 42 to 48.
The stemline chromosome number is hyperdiploid with the 2S component occurring at 3.8%. Twenty-one markers were common to nearly all S metaphases studied. Of these, 13 were two-armed chromosomes, and eight, acrocentric. Four markers were isochromosomes by Robertsonian translocations from chromosomes 4, 12, 14 and 15. Normal X, 12, 14, 15 and 16 were absent. The X chromosome was present as an arm of Rb(X.3). (See Cancer Genet. Cytogenet. 24: 95-107, 1987 for a detailed karyotype description.) Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.
Clinical Data
male
Antigen Expression
H-2k
Tumorigenic Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10exp7 cells
Effects
Yes, in nude mice
Comments

The cells are 5-bromo-2'-deoxyuridine resistant (BUDR, BrdUrd) and thymidine kinase (TK) deficient, HAT sensitive.

Tested and found negative for ectromelia virus (mousepox).


Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Atmosphere:  air, 95%; carbon dioxide (CO2), 5%
Name of Depositor TR Chen
References

Kit S, et al. Deletion of thymidine kinase activity from L cells resistant to bromodeoxyuridine. Exp. Cell Res. 31: 297-312, 1963. PubMed: 14056989

Chen TR. Cytogenetics of somatic cell hybrids. II. Modal karyotypes and mosaicism in mouse mutant cell lines: LM(tk-), A9, and Rag. Cancer Genet. Cytogenet. 24: 95-107, 1987. PubMed: 3466673

Hsu TC, Somers CE. Properties of L cells resistant to 5-bromodeoxyuridine. Exp. Cell Res. 26: 404-410, 1962. PubMed: 14449548

Boone C, et al. Assignment of three human genes to chromosomes (LDH-A to 11, TK to 17, and IDH to 20) and evidence for translocation between human and mouse chromosomes in somatic cell hybrids (thymidine kinase-lactate dehydrogenase A-isocitrate dehydrogenase-C-11, E-17, and F-20 chromosomes). Proc. Natl. Acad. Sci. USA 69: 510-514, 1972. PubMed: 4110482

Peterson JL, McBride OW. Cotransfer of linked eukaryotic genes and efficient transfer of hypoxanthine phosphoribosyltransferase by DNA-mediated gene transfer. Proc. Natl. Acad. Sci. USA 77: 1583-1587, 1980. PubMed: 6929511

Higazi AA, et al. Soluble human urokinase receptor is composed of two active units. J. Biol. Chem. 272: 5348-5353, 1997. PubMed: 9030610

Carballo E, et al. Bone marrow transplantation reproduces the tristetraprolin-deficiency syndrome in recombination activating gene-2 (-/-) mice. J. Clin. Invest. 100: 986-995, 1997. PubMed: 9276715

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Kit S, et al. Deletion of thymidine kinase activity from L cells resistant to bromodeoxyuridine. Exp. Cell Res. 31: 297-312, 1963. PubMed: 14056989

Chen TR. Cytogenetics of somatic cell hybrids. II. Modal karyotypes and mosaicism in mouse mutant cell lines: LM(tk-), A9, and Rag. Cancer Genet. Cytogenet. 24: 95-107, 1987. PubMed: 3466673

Hsu TC, Somers CE. Properties of L cells resistant to 5-bromodeoxyuridine. Exp. Cell Res. 26: 404-410, 1962. PubMed: 14449548

Boone C, et al. Assignment of three human genes to chromosomes (LDH-A to 11, TK to 17, and IDH to 20) and evidence for translocation between human and mouse chromosomes in somatic cell hybrids (thymidine kinase-lactate dehydrogenase A-isocitrate dehydrogenase-C-11, E-17, and F-20 chromosomes). Proc. Natl. Acad. Sci. USA 69: 510-514, 1972. PubMed: 4110482

Peterson JL, McBride OW. Cotransfer of linked eukaryotic genes and efficient transfer of hypoxanthine phosphoribosyltransferase by DNA-mediated gene transfer. Proc. Natl. Acad. Sci. USA 77: 1583-1587, 1980. PubMed: 6929511

Higazi AA, et al. Soluble human urokinase receptor is composed of two active units. J. Biol. Chem. 272: 5348-5353, 1997. PubMed: 9030610

Carballo E, et al. Bone marrow transplantation reproduces the tristetraprolin-deficiency syndrome in recombination activating gene-2 (-/-) mice. J. Clin. Invest. 100: 986-995, 1997. PubMed: 9276715