alpha-ACE 3.1.1 [ACE 3.1.1, a-ACE 3.1.1] (ATCC® HB-8191)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with purified ACE and endothelial cells of mouse, bovine and human origin.
The antibody can be used to isolate endothelial cells from the above species.
Derivation
Spleen cells were fused with P3-NSI/1-Ag4/1 myeloma cells.
The antibody reacts with purified ACE and endothelial cells of mouse, bovine and human origin.
Genes Expressed
immunoglobulin; monoclonal antibody; against angiotensin converting enzyme (ACE)
Cellular Products
immunoglobulin; monoclonal antibody; against angiotensin converting enzyme (ACE)
Tumorigenic Yes
Effects
Yes, forms ascites in pristane primed BALB/cAu mice
Comments
Animals were immunized with a crude preparation of rat lung ACE with incomplete Freund's adjuvant.
Spleen cells were fused with P3-NSI/1-Ag4/1 myeloma cells.
The antibody reacts with purified ACE and endothelial cells of mouse, bovine and human origin.
The antibody can be used to isolate endothelial cells from the above species.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose and 0.005 mM 2-mercaptoethanol, 85%; heat-inactivated fetal bovine serum, 15%
Complete Growth Medium
Culture Medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine
adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, and 1.0 mM
sodium pyruvate, supplemented with 50 ?M 2-mercaptoethanol, 90%; fetal bovine
serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgM
Name of Depositor Wisconsin Alumni Res. Fndn.
References

Auerbach R, et al. Monoclonal antibody against angiotensin-converting enzyme: Its use as a marker for murine, bovine, and human endothelial cells. Proc. Natl. Acad. Sci. USA 79: 7891-7895, 1982. PubMed: 6296854

Auerbach R. Monoclonal antibody to angiotension-converting enzyme and methods of preparing and using same. US Patent 4,582,790 dated Apr 15 1986

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Auerbach R, et al. Monoclonal antibody against angiotensin-converting enzyme: Its use as a marker for murine, bovine, and human endothelial cells. Proc. Natl. Acad. Sci. USA 79: 7891-7895, 1982. PubMed: 6296854

Auerbach R. Monoclonal antibody to angiotension-converting enzyme and methods of preparing and using same. US Patent 4,582,790 dated Apr 15 1986