127H (ATCC® HB-11911)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Strain

Strain: BALB/c (B cell); BALB/c (myeloma)

Derivation
Mice were immunized with the purified recombinant rat ICAM-4, domains 1-6 protein.
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against rat ICAM-4
Cellular Products
immunoglobulin; monoclonal antibody; against rat ICAM-4
Comments
Mice were immunized with the purified recombinant rat ICAM-4, domains 1-6 protein.
Spleen cells were fused with NS-1 myeloma cells.
Monoclonal antibody 127A (ATCC HB-11905) recognized the D1/GST fusion protein and 127H (ATCC HB-11911) recognized the D2/GST fusion protein.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
Subculturing
Maintain cultures at cell concentrations between 2 X 10exp5 and 6 X 10exp5 viable cells/ml.
Culture Conditions
Temperature: 37.0°C
Isotype IgG1
Name of Depositor ICOS Corporation
References

Kilgannon PD, Gallatin M. Anti-ICAM-4 antibodies and hybridomas. US Patent 5,773,293 dated Jun 30 1998

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Kilgannon PD, Gallatin M. Anti-ICAM-4 antibodies and hybridomas. US Patent 5,773,293 dated Jun 30 1998