Murine hepatitis virus (ATCC® VR-764)

Classification: Nidovirales, Coronaviridae, Coronavirus,Group 2  /  Product Format: frozen

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Classification Nidovirales, Coronaviridae, Coronavirus,Group 2
Agent Murine hepatitis virus
Strain MHV-A59
Common Name Murine hepatitis virus
Biosafety Level 2
Isolation
Pool of liver and spleen suspension of BALB/c mice exhibiting rough coats and hunched position
Product Format frozen
Storage Conditions -70°C or colder (or colder)
Comments
The MHV-A59 virus was isolated from BALB/c mice in which Moloney murine leukemia had been serially passaged by i.p. injection of homogenates of liver, spleen, thymus and lymph node. It is possible that the preparation still contains murine leukemia virus.
Effect on Host
Yes, in vitro effects: Cytopathic effects in tissue culture
CPE, rounding and sloughing, attached and floating syncytial debris
Growth Medium NCTC 135 medium, 90%; horse serum, 10%
Recommended Host
NCTC clone 1469 cells (ATCC CCL-9.1)

Recommended Host:  NCTC 1469 (ATCC CCL-9.1)

Growth Conditions
Incubation: 18 to 24 hours at 37°C, a 5%CO2 in air atmosphere is recommended
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 80% - 90% confluent (not 100% confluent).
Temperature: 37°C
Cryopreservation
Storage Temperature: -70°C or colder
Effect on Host
Yes, in vitro effects: Cytopathic effects in tissue culture
CPE, rounding and sloughing, attached and floating syncytial debris
Name of Depositor MJ Collins
Special Collection ATCC
Source
Pool of liver and spleen suspension of BALB/c mice exhibiting rough coats and hunched position
References

Piczak CV, et al. A hepatitis virus complicating studies with mouse leukemia. J. Natl. Cancer Inst. 27: 29-51, 1961. PubMed: 13766009

rounding and sloughing with attached and floating syncytial debris

or colder

or colder

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation