VR-764 ™
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
The MHV-A59 virus was isolated from BALB/c mice in which Moloney murine leukemia had been serially passaged by i.p. injection of homogenates of liver, spleen, thymus and lymph node. It is possible that the preparation still contains murine leukemia virus.
Next-generation sequencing (NGS) at ATCC on the NCTC clone 1469 cell line (ATCC CCL-9.1) used as the host has shown the presence of Murine leukemia virus sequences.
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Piczak CV, et al. A hepatitis virus complicating studies with mouse leukemia. J. Natl. Cancer Inst. 27: 29-51, 1961. PubMed: 13766009
rounding and sloughing with attached and floating syncytial debris
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