HCC38 (ATCC® CRL-2314)

Organism: Homo sapiens, human  /  Cell Type: Epithelial  /  Tissue: mammary gland; breast/duct  /  Disease: TNM stage IIB, grade 3, primary ductal carcinoma

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Organism Homo sapiens, human
Tissue mammary gland; breast/duct
Cell Type Epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent, single cells and loosely attached clusters
Biosafety Level 1
Disease TNM stage IIB, grade 3, primary ductal carcinoma
Age 50 years adult
Gender female
Ethnicity Caucasian, White
Storage Conditions liquid nitrogen vapor phase
Karyotype Number of cells examined = 59; Modal Chromosome Number = 75 with a range of 65 to 79; Polyploidy Rate = 22% HCC38 contains a homozygous deletion at 3p12. At least 45 distinct derivative chromosomes were detected in most metaphases, including two large metacentric markers which are approximately 1.5 times longer than a normal A group chromosome. Other derivative chromosomes: del(1)(q24); del(1)(p22); del(1)(p13); add(1)(p12); del(2)(p16); add(3)(q10); del(3)(q13); ?del(4)(q13) (two copies per cell); del(5)?(q23q33); der (7)(pterÕq11::?hsr); del(7)(p?); del(9)(p12); add(9)(p10); ?der(11); add(12)(q24)(very long); add(14)(p11); add(17)(p12); der(18); der(X); plus approx. 17-24 markers of unknown origin per cell. This is a hyper-triploid human cell line with a modal chromosome number of 75. Homogeneously staining regions and dicentric chromosomes were observed. Every chromosome pair had a least one rearrangement. No normal X chromosomes were observed and Y chromosomes were absent by QM staining. The following structural rearrangements were observed in 30 metaphases: an acentric fragment in 2/30 metaphases, a minute in 3/30, a chromosome break in 3/30, a chromatid break in 5/30, a ring chromosome in 1/30, and double minutes in 11/30 (1-5 copies). Pulverized chromosomes were reported in 5% of metaphases. C-banding revealed that several of the large markers are dicentric. No normal X chromosomes were observed and Y chromosomes were absent by QM staining. Normal copies of chromosomes 2,6,11,13,16 and 20 were seen. Composite karyotype: 65-79<3n> der(X), -1,-1,-1, del(1)(q24), del(1)(p22),del(1)(p13),add(1)(p12),-2,del(2)(p16)x2,-3,-3,-3, ?add(3)(q10),del(3)(q13)x2,-4,-4,-4, ?del(4)(q13)x2,-5, -5,-5,del(5)?(q23q33)x2,-6,-6,-7,-7,-7,der (7)(pterÕq11::?hsr), del(7)(p?)x2,-8,-8,-8,-9,-9,-9,del(9)(p12)x2,add(9)(p10)x2, -10,-10,-10,-11,?der(11),-12,-12,-12, add(12)(q24), -13,-13,-14,-14,-14,add(14)(p11)x2,-15,-15,-15,-17,-17,-17, add(17)(p12)x2,-18,-18,-18,der(18),-19,-19,-19,-21,-21, -22,-22,+17 to 24 mar[cp11].
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Derivation
HCC38 was initiated from a 50-year-old white female with a prior history of leiomyosarcoma; her mother died of breast cancer.
This cell line was initiated on 4/27/92 and took 32 months to establish.
Clinical Data
50 years
Caucasian, White
female

Receptor Expression
estrogen receptor, not expressed
progesterone receptor, not expressed
Oncogene her2/neu -; p53 +
Genes Expressed
Epithelial glycoprotein 2 [EGP2]; cytokeratin 19
Comments

HCC38 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 [EGP2] and for cytokeratin 19, and is negative for expression of estrogen receptor (ER) and progesterone receptor (PR).

The cells are negative for expression of Her2-neu but positive for expression of p53

The tumor was classified as TMN Stage IIB, Grade 3, with 3/28 lymph node metastasis.


Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.


Phenol Red is a structural mimic for estrogen. Therefore the estrogenic activity of phenol red should be considered in any studies that utilize estrogen-responsive cells in culture (RefBerthois Y, et al. Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture. Proc. Natl. Acad. Sci. USA 83: 2496–2500, 1986. PubMed: 3458212). The Phenol Red-free version of RPMI 1640, is ATCC catalog No. 30-2602.

Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C
Subculture Ratio: 1:2 to 1:4
Medium Renewal: Every 2 to 3 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 12,14
D16S539: 10,14
D5S818: 9
D7S820: 10
THO1: 9.3
TPOX: 9,12
vWA: 16,17
Name of Depositor AF Gazdar, AK Virmani
Year of Origin April 27, 1992
References

Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771

Sundarsan V, et al. Homozygous deletions at 3p12 in breast and lung cancer. Oncogene 17: 1723-1729, 1998. PubMed: 9796701

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
Restrictions

The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 648-1888, Email TechnologyDevelopment@UTSouthwestern.edu, or Fax: (214) 951-0935.

References

Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771

Sundarsan V, et al. Homozygous deletions at 3p12 in breast and lung cancer. Oncogene 17: 1723-1729, 1998. PubMed: 9796701