Plasmodium falciparum Welch (ATCC® 50005)

Organism: Plasmodium falciparum Welch  /  Depositor: W Trager

Strain Designations FCR-3/Gambia Subline F-86
Application
Emerging infectious disease research
Vector borne research
Biosafety Level 2
Isolation
derived from ATCC 30932
Product Format frozen
Type Strain no
Antibiotic Resistance
Chloroquine resistant.
Comments
Selected for knobby trait.
Chloroquine resistant.
pyrimethamine-sensitive
Medium ATCC® Medium 2196: Malaria medium, complete
Growth Conditions
Temperature: 37.0°C
Growth condition: in vitro culture in human erythrocytes. Consult product sheet for protocol.
Cryopreservation

CRYOPRESERVATION: 

Only young cells (rings) can be frozen in glycerolyte medium** because their membranes are more robust.

1.     Centrifuge ring-stage culture for 5 mins. at 1000 x g in 50 ml centrifuge tube.

2.     Aspirate supernatant using sterile Pasteur pipet.

3.     Resuspend pellet gently in remaining supernatant.

4.     Slowly add 5 volumes of glycerolyte medium to 3 volumes pellet dropwise via a syringe as follows:

        A.    Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature.

B.    Add the remaining 4 volumes of glycerolyte and gently agitate.

5.   Aliquot mixture into Nunc screw-capped freezing vials and place in a Nalgene 1C cooling apparatus. Place the apparatus at -80°C overnight and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately -1°C/min.).

6.   Plunge vials into liquid nitrogen (-196oC) the next day and store in liquid nitrogen or liquid nitrogen vapor.

       

Name of Depositor W Trager
Special Collection ATCC
References

Jensen JB, Trager W. Plasmodium falciparum in culture: establishment of additional strains. Am. J. Trop. Med. Hyg. 27: 743-746, 1978. PubMed: 356635