Plasmodium cynomolgi Mayer (ATCC® 30149)

Strain Designations: Gombak  /  Depositor: National Institutes of Health  /  Biosafety Level: 2

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Strain Designations Gombak
Application
Vector borne research
Biosafety Level 2
Isolation
Anopheles balabacensis introlatus, West Malaysia, 1963
Product Format frozen
Type Strain no
Comments
This material is not available for international distribution due to the lack of historical data to support the issuance of a U.S. Fish and Wildlife Service Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES) export permit.
Growth Conditions
Duration: in vivo, Macaca mulatta
Protocol: ATCCNO: 30037 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the strain arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. When it is completely thawed, aseptically remove the material with a syringe and inject the entire contents of the vial into an Aotus trivirgatus. This parasite can be maintained by serial passage of parasitized blood (by intravenous inoculation) into monkeys (Aotus trivirgatus).
Subcultivation
Protocol: ATCCNO: 30037 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the strain arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. When it is completely thawed, aseptically remove the material with a syringe and inject the entire contents of the vial into an Aotus trivirgatus. This parasite can be maintained by serial passage of parasitized blood (by intravenous inoculation) into monkeys (Aotus trivirgatus).
Name of Depositor National Institutes of Health
Chain of Custody
ATCC <<--National Institutes of Health<<--D.E. Eyles
Year of Origin 1963
References

Eyles DE, et al. Identification of Anopheles balabacensis introlatus as a vector of monkey malaria in Malaya. Bull. World Health Organ. 28: 134-135, 1963. PubMed: 14166986

Coatney GR. The primate malarias. Washington, DC: Government Printing Office; 1971.

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