H9 [derivative of HuT 78] (ATCC® HTB-176)

Organism: Homo sapiens, human  /  Cell Type: cutaneous T lymphocyte  /  Disease: lymphoma

Organism Homo sapiens, human
Cell Type cutaneous T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease lymphoma
Age 53 years
Gender Male
Ethnicity Caucasian
Applications

This cell line is a suitable transfection host.

Storage Conditions liquid nitrogen vapor phase
Karyotype

This is a near triploid cell line (modal number = 69; range = 58 to 74). The frequency of higher ploidies is 2.5%. The line has an extremely complex karyotype with nearly 60% of the chromosomes in each cell being structurally altered marker chromosomes., Among the markers are t(3p4q), t(5q6q), t(5p6p), i(18q), i(18p); t(4q7p), and del(7)(q32). The first four of these are usually paired. Normal N4, N5, N6, N7, N10, N13, N18, N19, N20 and X are absent.

Images
Derivation
The H9 cell line is a clonal derivative of the Hut 78 cell line (see ATCC TIB-161).
Clinical Data
53 years
Caucasian
male
The H9 clone was selected for permissiveness for HIV-1 replication, and has been used to isolate and propagate HIV-1 from the blood of patients with acquired immunodeficiency syndrome (AIDS) and pre-AIDS conditions.
Antigen Expression
CD4; HLA A1, B62, C3, DR4, DQ3
Receptor Expression Interleukin 2 (IL-2)
Genes Expressed
interleukin-2 (interleukin 2, IL-2), CD4, HLA A1, B62, C3, DR4, DQ3
Tumorigenic Yes
Effects
Yes, in nude mice inoculated subcutaneously with 10(7) cells
Virus Susceptibility Human immunodeficiency virus 1 , Human immunodeficiency virus 1
Comments
The H9 clone was selected for permissiveness for HIV-1 replication, and has been used to isolate and propagate HIV-1 from the blood of patients with acquired immunodeficiency syndrome (AIDS) and pre-AIDS conditions.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 105 viable cells/mL.
Maintain cultures at cell concentrations between 5 x 105 and 2 x 106 viable cells/mL.
Do not allow cell concentration to exceed 3 X 106 cells/mL.
Medium Renewal: Every 2 to 4 days
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11
D13S317: 8,12
D16S539: 11,12
D5S818: 11
D7S820: 8,11
THO1: 8,9
TPOX: 8,9
vWA: 14,15
Isoenzymes
AK-1, 0
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 0
PGM1, 1
PGM3, 0
Name of Depositor RC Gallo, M Popovic
References

Gootenberg JE, et al. Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor. J. Exp. Med. 154: 1403-1418, 1981. PubMed: 6975346

Mann DL, et al. Origin of the HIV-susceptible human CD4+ cell line H9. AIDS Res. Hum. Retroviruses 5: 253-255, 1989. PubMed: 2567177

Gazdar AF, et al. Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas. Blood 55: 409-417, 1980. PubMed: 6244013

Popovic M, et al. Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS. Science 224: 497-500, 1984. PubMed: 6200935

Chen TR. Karyotypic derivation of H9 cell line expressing human immunodeficiency virus susceptibility. J. Natl. Cancer Inst. 84: 1922-1926, 1992. PubMed: 1460674

Popovic M, et al. T4 positive human neoplastic cell lines susceptible to and permissive for HTLV-III. Lancet 2: 1472-1473, 1984. PubMed: 6151082

Brigino E, et al. Interleukin 10 is induced by recombinant HIV-1 Nef protein involving the calcium/calmodulin-dependent phosphodiesterase signal transduction pathway. Proc. Natl. Acad. Sci. USA 94: 3178-3182, 1997. PubMed: 9096366

Anderson SM, et al. Intercellular transfer of a glycoslphosphatidylinositol (GPI)-linked protein: release and uptake of CD4-GPI from recombinant adeno-associated virus-transducted HeLa cells. Proc. Natl. Acad. Sci. USA 93: 5894-5898, 1996. PubMed: 8650189

Tumors developed within 21 days at 100% frequency (5/5).

Basic Documentation
Other Documentation
References

Gootenberg JE, et al. Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor. J. Exp. Med. 154: 1403-1418, 1981. PubMed: 6975346

Mann DL, et al. Origin of the HIV-susceptible human CD4+ cell line H9. AIDS Res. Hum. Retroviruses 5: 253-255, 1989. PubMed: 2567177

Gazdar AF, et al. Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas. Blood 55: 409-417, 1980. PubMed: 6244013

Popovic M, et al. Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS. Science 224: 497-500, 1984. PubMed: 6200935

Chen TR. Karyotypic derivation of H9 cell line expressing human immunodeficiency virus susceptibility. J. Natl. Cancer Inst. 84: 1922-1926, 1992. PubMed: 1460674

Popovic M, et al. T4 positive human neoplastic cell lines susceptible to and permissive for HTLV-III. Lancet 2: 1472-1473, 1984. PubMed: 6151082

Brigino E, et al. Interleukin 10 is induced by recombinant HIV-1 Nef protein involving the calcium/calmodulin-dependent phosphodiesterase signal transduction pathway. Proc. Natl. Acad. Sci. USA 94: 3178-3182, 1997. PubMed: 9096366

Anderson SM, et al. Intercellular transfer of a glycoslphosphatidylinositol (GPI)-linked protein: release and uptake of CD4-GPI from recombinant adeno-associated virus-transducted HeLa cells. Proc. Natl. Acad. Sci. USA 93: 5894-5898, 1996. PubMed: 8650189

Tumors developed within 21 days at 100% frequency (5/5).