J.RT3-T3.5

 J.RT3-T3.5 cells lack the beta chain of the T cell antigen receptor.

The cells do not express either CD3 or the T cell receptor alpha/beta heterodimer on the surface.

The cells may be useful for transfection studies involving the T cell receptor beta chain gene.

1. Check all containers for leakage or breakage.
2. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below ­-130°C, preferably in liquid nitrogen vapor, until ready for use.

1. Initiate culture as soon as possible upon receipt.
2. Thaw by rapid agitation in 37°C water bath. Thawing should be rapid (within 40-60 seconds). As soon as the ice is melted, remove the ampule from the water bath and immerse in 70% ethanol at room temperature. All of the operations from this point on should be carried out under strict aseptic conditions.
3. Transfer the cell suspension and dilute it with the recommended culture medium in a culture flask (see specific batch information above for dilution ratio); incubate at 37°C with 5% CO₂ in air atmosphere. Since it is important to avoid excessive alkalinity of the medium during recovery of the cells, it is suggested that the culture medium be placed into the culture flask, tube, etc. and the pH be adjusted, as necessary, prior to the addition of the ampule contents. Note that the bicarbonate content of the culture medium will determine whether an atmosphere containing CO₂ will be required.
4. It is not necessary to remove the freezing additive. However, if desired, the culture medium may be changed to remove the protective freezing additive (dimethylsulfoxide) 24 hours after thawing. If it is desired that the freezing additive be removed immediately, or that a more concentrated cell suspension be obtained, centrifuge the above diluted suspension at approximately 125 x g for 10 minutes, discard the fluid and resuspend the cells with growth medium at the dilution ratio given in the specific batch information above

FLUID RENEWAL

Every 2-3 days.

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.