Geobacillus stearothermophilus (Donk) Nazina et al. (ATCC® 10149)

Strain Designations: NRS T15 [JCM 11297]  /  Type Strain: no  /  Biosafety Level: 1

Deposited As Bacillus calidolactis Hussong and Hammer
Strain Designations NRS T15 [JCM 11297]
Application
Assay of lactam antibiotics beta-lactam antibiotics
This strain is recommended by ATCC for use in the tests described in AOAC Official Methods 982.15, 982.18, and 988.08 where only the taxon is specified.
Food testing
Type Strain no
Biosafety Level 1
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Intended Use
Preceptrol® yes
Type Strain no
Medium ATCC® Medium 3: Nutrient agar or nutrient broth
Growth Conditions
Temperature: 55°C
Atmosphere: Aerobic
Name of Depositor NR Smith
Chain of Custody
ATCC <-- NR Smith <-- Curran <-- Iowa State College
References

Smith NR, et al. Aerobic spore forming bacteria. U.S. Dep. Agric. Monogr. 16: 1-148, 1952.

AOAC International Beta-lactam antibiotics, qualitative disc method I. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 979.14.

AOAC International Beta-lactam antibiotics in milk, quantitative disc method. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 982.16.

AOAC International Beta-lactam antibiotics in milk, qualitative disc method II. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 982.17.

U.S. Food & Drug AdministrationInhibitory substances in milk, Bacillus stearothermophilus disk assay -- Qualitative method IIn: U.S. Food & Drug AdministrationBacteriological analytical manual8th ed.Gaithersburg, MDAOAC Internationalpp. 20.04-20.06, 1995

Inhibitory Substance in Milk. Washington, DC:US Food & Drug Administration;Bacteriological Analytical Manual Online Chapt. 20A, 2001

ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions)

Corry, JEL, Curtis, GDW and Baird, RM (Eds) Handbook of Culture Media for Food and Water Microbiology. Royal Society of Chemistry, In preparation. ICFMH-WPCM.