Orientia chuto (ATCC® VR-1744)

Classification: Rickettsiaceae, Orientia  /  Product Format: frozen

Permits and Restrictions

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Classification Rickettsiaceae, Orientia
Agent Orientia chuto
Strain Dubai
Biosafety Level 3
Product Format frozen
Storage Conditions  -70°C or colder
Comments
New Taxon.
It is recommended to determine viability by a PCR-based assay as agent does not produce cytopathic effects.
Effect on Host
No CPE produced
Recommended Host
Vero cells (ATCC CCL-81)

Production Host:  Vero cells (ATCC® CCL-81™)

Growth Conditions
Temperature: 35°C
Recommendations for Infection: Plant cells 24 hours in advance and infect when cultures are 90-100% confluent. Remove medium and inoculate with an appropriate volume of Rickettsiae (e.g. 1 mL per 25 cm2) diluted in agent growth medium to provide an appropriate MOI (e.g. MOI of 50 for 50 Rickettsiae/ cell). Adsorb 1 hour at 800 x g at 20?C in a low speed centrifuge in closed buckets. End adsorption by adding virus growth medium to culture(s).
Incubation: Incubate infected culture(s) for 7-14 days at 35°C in a humidified 5% CO2 atmosphere.
Effect on Host
No CPE produced
Name of Depositor L Izzard
Source
Clinical female patient with acute scrub typhus infection, Dubai, United Arad Emirates, 2006.
References

Izzard L, et. al. Isolation of a novel Orientia species (O. chuto sp. nov.) from a patient infected in Dubai. J. Clin. Micro. 48(12): 4404-4409, 2010.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation