Forms intracellular inclusions which react with specific anti-C. pneumoniae monoclonal antibodies and monoclonal antibodies directed against the chlamydia LPS (genus antigen: Kallestad C. trachomatis Culture Confirmation System).
Will not stain with iodine or monoclonal antibodies against the MOMP of C. trachomatis. Infection is enhanced by centrifugation and use of cycloheximide (1-2ug/ml) in medium.
This preparation has tested positive for Mycoplasma contamination.
Infection is enhanced by centrifugation and use of cycloheximide (1-2ug/ml) in medium.
Effect on Host
Cytopathic effects (intracellular inclusions) in tissue culture which may be visualized by appropriate conjugated antibodies
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated antibodies.