Simian virus 40 (ATCC® VRMC-5)

Classification: Polyomaviridae, Polyomavirus  /  Product Format: frozen

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Classification Polyomaviridae, Polyomavirus
Agent Simian virus 40
Strain pUCSV-VA45-54-1Ea [molecular clone of Simian virus 40, SV-40]
Common Name Simian virus 40
Applications
contains sequence
Biosafety Level 2
Product Format frozen
Comments
Insert contains restriction sites from 5'end: BamHI-0.771,7.086; EcoRI-2.686,5.171;KpnI-3.696,4.161; SalI-7.857; SfiI-7.857; XhoI-uncut. The plasmid size = 7857 bp [2686bp(pUC-19 vector) +5171bp(SV40-VA45-54-1insert)].
The variant of SV-40 strain VA-45-54 has an archetypal viral regulatory region and was verified by lipofection of EcoRI-linearized plasmid pUCSV-VA45-54-1Ea DNA into ATCC CV-1 cells by the contributor.
Growth Medium ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
Name of Depositor JA Lednicky, JS Butel
Special Collection NCRR Contract
References

Lednicky JA, Butel JS. Tissue culture adaptation of natural isolates of simian virus 40: changes occur in viral regulatory region but not in carboxy-terminal domain of large T-antigen. J. Gen. Virol. 78: 1697-1705, 1997. PubMed: 9225047

Stewart AR, et al. Sequence analyses of human tumor-associated SV40 DNAs and SV40 viral isolates from monkeys and humans. J. Neurovirol. 4: 182-193, 1998. PubMed: 9584955

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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