pUCSV40-CPC/MEN-1 [molecular clone of Simian virus 40, SV-40] (ATCC® VRMC-4)

Organism: Simian virus 40, Simian virus 40  /  Clone Type: Clone  /  Depositors: JA Lednicky, JS Butel

Permits and Restrictions
Permits and Restrictions

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Designations pUCSV40-CPC/MEN-1 [molecular clone of Simian virus 40, SV-40]
GenBank Number

AF156108

Species Simian virus 40, Simian virus 40
Depositors JA Lednicky, JS Butel
Isolation
Contains a full-length genome of SV-40 strain CPC/MEN cloned into the EcoRI site of pUC-19. SV40-CPC/MEN was isolated and cloned from virus stock derived from choroid plexus carcinoma DNA sample 12 (from a 4 yr old child).
Applications
contains sequence
Vector
Construct size (kb): 7.866000175476074

DESCRIPTION OF VECTOR COMPONENT

        Type of vector:  E. coli

        Name of vector: pUC-19

        Intact vector size (kb):  2.686 kb

Insert
DNA: cDNA
Insert lengths(kb): 5.179999828338623
Tissue: choroid plexus carcinoma
Gene product: [SV40-CPC/MEN-1]
Insert Size (kb) 5.180
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 2
Comments
It has an archetypal viral regulatory region and was tested by lipofection of EcoRI-linearized plasmid DNA into TC7 cells, with cells showing subsequent production of typical SV-40 CPE. Lipofection into TC7 and Butel lab CV-1 cells was verified.
Contains a full-length genome of SV-40 strain CPC/MEN cloned into the EcoRI site of pUC-19. SV40-CPC/MEN was isolated and cloned from virus stock derived from choroid plexus carcinoma DNA sample 12 (from a 4 yr old child).
Classification Polyomaviridae, Polyomavirus
References

Stewart AR, et al. Sequence analyses of human tumor-associated SV40 DNAs and SV40 viral isolates from monkeys and humans. J. Neurovirol. 4: 182-193, 1998. PubMed: 9584955

Lednicky JA, et al. Natural simian virus 40 strains are present in human choroid plexus and ependymoma tumors. Virology 212: 710-717, 1995. PubMed: 7571441

Product Sheet
Product Sheet
Product Sheet