pUCSV40-CPC/MEN-1 [molecular clone of Simian virus 40, SV-40] (ATCC® VRMC-4)

Organism: Simian virus 40, Simian virus 40  /  Clone Type: Clone  /  Depositors: JA Lednicky, JS Butel

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Designations pUCSV40-CPC/MEN-1 [molecular clone of Simian virus 40, SV-40]
GenBank Number

AF156108

Species Simian virus 40, Simian virus 40
Depositors JA Lednicky, JS Butel
Isolation
Contains a full-length genome of SV-40 strain CPC/MEN cloned into the EcoRI site of pUC-19. SV40-CPC/MEN was isolated and cloned from virus stock derived from choroid plexus carcinoma DNA sample 12 (from a 4 yr old child).
Applications
contains sequence
Vector
Construct size (kb): 7.86

DESCRIPTION OF VECTOR COMPONENT

        Type of vector:  E. coli

        Name of vector: pUC-19

        Intact vector size (kb):  2.68 kb

Insert
DNA: cDNA
Insert lengths(kb): 5.179999828338623
Tissue: choroid plexus carcinoma
Gene product: [SV40-CPC/MEN-1]
Insert Size (kb) 5.180
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
It has an archetypal viral regulatory region and was tested by lipofection of EcoRI-linearized plasmid DNA into TC7 cells, with cells showing subsequent production of typical SV-40 CPE. Lipofection into TC7 and Butel lab CV-1 cells was verified.
Contains a full-length genome of SV-40 strain CPC/MEN cloned into the EcoRI site of pUC-19. SV40-CPC/MEN was isolated and cloned from virus stock derived from choroid plexus carcinoma DNA sample 12 (from a 4 yr old child).
Classification Polyomaviridae, Polyomavirus
References

Stewart AR, et al. Sequence analyses of human tumor-associated SV40 DNAs and SV40 viral isolates from monkeys and humans. J. Neurovirol. 4: 182-193, 1998. PubMed: 9584955

Lednicky JA, et al. Natural simian virus 40 strains are present in human choroid plexus and ependymoma tumors. Virology 212: 710-717, 1995. PubMed: 7571441

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