Simian virus 40 (ATCC® VRMC-35)

Classification: Polyomaviridae, Polyomavirus  /  Product Format: frozen

Permits and Restrictions

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Classification Polyomaviridae, Polyomavirus
Agent Simian virus 40
Strain pUCSV40-777-5 [molecular clone of Simian virus 40, SV-40]
Common Name Simian virus 40
Applications
contains sequence
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Description of cloned agent:
Product Format frozen
Comments
The insert contains the following restriction sites (kb): BamHI--4.549, 3.417; EcoRI--5.215, 2.686; KpnI--6.365, 1.591; SalI--7.956; SfiI--7.956; XhoI--uncut
Description of cloned agent:
Name: SV40
Classification: Polyomavirus
Plasmid copy#: High-copy number (pUC19 has a ColEI ori)
Viable virus clone. Verified by lipofection of EcoRI-linearized plasmid DNA into ATCC CV-1 cells (passage# 40) by J. Lednicky (lipofection date: 12 Oct. 2000).
The viral regulatory region of this construct is complex (non-archetypal) but distinct from the complex regulatory region of SV40-777-5. Plasmid size: 7.956 kb [2.686 kb (pUC19 vector) + 5.270 kb (SV40-777-5 insert)]
Name of Depositor JA Lednicky
Special Collection NCRR Contract
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation