Simian virus 40 (ATCC® VRMC-11)

Organism: Simian virus 40, Simian virus 40  /  Clone Type: Clone  /  Depositors: JA Lednicky, JS Butel

Permits and Restrictions

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Designations pUCSVH388-16 [molecular clone of Simian virus 40, SV-40]
Species Simian virus 40, Simian virus 40
Depositors JA Lednicky, JS Butel
Isolation
Description of cloned agent:
Contains a full-length non-archetypal SV40-H388 genome cloned into pUC-19 at EcoRI site; isolated from peripheral blood mononuclear cell DNA of animal H388 by J. Lednicky.
Applications
contains sequence
Vector
Construct size (kb): 7.89300012588501

DESCRIPTION OF VECTOR COMPONENT

        Type of vector:  E. coli

        Name of vector:  pUC-19

        Intact vector size (kb):  2.686 kb

Insert
DNA: genomic
Insert lengths(kb): 5.206999778747559
Tissue: monkey peripheral blood mononuclear cell
Insert Size (kb) 5.207
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 2
Comments
Description of cloned agent:
Name: SV40
Strain: H388, variant #16
Classification: Papovavirus
Contains a full-length non-archetypal SV40-H388 genome cloned into pUC-19 at EcoRI site; isolated from peripheral blood mononuclear cell DNA of animal H388 by J. Lednicky.
Plasmid size: 7893 bp [pUC-19 (vector): 2686 bp + SV40-H388 (insert): 5207 bp]. High-copy number; pUC-19 has a ColEI ori.
VRMC-11 (variant #16) is one of a matched set of 3 clones of regulatory region variants (see also VRMC-9 and VRMC-10).
Plasmid might dimerize; restriction pattern will not change.
Restriction enzyme analysis: BamHI--4.479,3.414; EcoRI--5.207,2.686; KpnI--6.392,1.501; SalI--7.893; SfiI--7.893; XhoI--uncut.
Sequence (viral regulatory region, T-ag carboxy terminus, and part of VP1 gene) determined by: Xian Min Dai. Clone viability: not tested.
Classification Polyomaviridae, Polyomavirus
References

Lednicky JA, et al. Natural isolates of simian virus 40 from immunocompromised monkeys display extensive genetic heterogeneity: new implications for polyomavirus disease. J. Virol. 72: 3980-3990, 1998. PubMed: 9557685

John A Lednicky, personal communication

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