Simian virus 40 (ATCC® VRMC-11™)

Classification: Polyomaviridae, Polyomavirus  /  Product Format: frozen

Classification Polyomaviridae, Polyomavirus
Agent Simian virus 40
Strain pUCSVH388-16 [molecular clone of Simian virus 40, SV-40]
Common Name Simian virus 40
Applications
contains sequence
Biosafety Level 2
Isolation
Description of cloned agent:
Contains a full-length non-archetypal SV40-H388 genome cloned into pUC-19 at EcoRI site; isolated from peripheral blood mononuclear cell DNA of animal H388 by J. Lednicky.
Product Format frozen
Comments
Description of cloned agent:
Name: SV40
Strain: H388, variant #16
Classification: Papovavirus
Contains a full-length non-archetypal SV40-H388 genome cloned into pUC-19 at EcoRI site; isolated from peripheral blood mononuclear cell DNA of animal H388 by J. Lednicky.
Plasmid size: 7893 bp [pUC-19 (vector): 2686 bp + SV40-H388 (insert): 5207 bp]. High-copy number; pUC-19 has a ColEI ori.
VRMC-11 (variant #16) is one of a matched set of 3 clones of regulatory region variants (see also VRMC-9 and VRMC-10).
Plasmid might dimerize; restriction pattern will not change.
Restriction enzyme analysis: BamHI--4.479,3.414; EcoRI--5.207,2.686; KpnI--6.392,1.501; SalI--7.893; SfiI--7.893; XhoI--uncut.
Sequence (viral regulatory region, T-ag carboxy terminus, and part of VP1 gene) determined by: Xian Min Dai. Clone viability: not tested.
Recommended Host
E. coli
Growth Conditions
Temperature: 37.0°C
Name of Depositor JA Lednicky, JS Butel
Special Collection NCRR Contract
References

Lednicky JA, et al. Natural isolates of simian virus 40 from immunocompromised monkeys display extensive genetic heterogeneity: new implications for polyomavirus disease. J. Virol. 72: 3980-3990, 1998. PubMed: 9557685

John A Lednicky, personal communication