pUCSVH388-2 [molecular clone of Simian virus 40, SV-40] (ATCC® VRMC-10)

Organism: Simian virus 40, Simian virus 40  /  Clone Type: Clone  /  Depositors: JA Lednicky, JS Butel

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Designations pUCSVH388-2 [molecular clone of Simian virus 40, SV-40]
Species Simian virus 40, Simian virus 40
Depositors JA Lednicky, JS Butel
Isolation
Description of cloned agent:
Contains a full-length non-archetypal SV40-H388 genome cloned into pUC-19 at EcoRI site; isolated from brain tissue (animal H388). Plasmid size: 7915 bp [pUC-19 (vector): 2686 bp + SV40-H388 (insert): 5229 bp]. High-copy number; pUC-19 has a ColEI ori.
Applications
contains sequence
Vector
Construct size (kb): 7.914999961853027

DESCRIPTION OF VECTOR COMPONENT

        Type of vector:  E. coli

        Name of vector:  pUC-19

        Intact vector size (kb):  2.686 kb

Insert
DNA: genomic
Insert lengths(kb): 5.229000091552734
Tissue: monkey brain
Insert Size (kb) 5.229
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Description of cloned agent:
Name: SV40
Strain: H388, variant #2
Classification: Papovavirus
Contains a full-length non-archetypal SV40-H388 genome cloned into pUC-19 at EcoRI site; isolated from brain tissue (animal H388). Plasmid size: 7915 bp [pUC-19 (vector): 2686 bp + SV40-H388 (insert): 5229 bp]. High-copy number; pUC-19 has a ColEI ori.
VRMC-10 (variant #2) is one of a matched set of 3 clones of regulatory region variants (see also VRMC-11 and VRMC-9).
Restriction enzyme analysis: BamHI--4.501,3.414; EcoRI--5.229,2.686; KpnI--6.414,1.501; SalI--7.915; SfiI--7.915; XhoI--uncut.
Sequence (viral regulatory region, T-ag carboxy terminus, and part of VP1 gene) determined by J. Lednicky. Viable clone; verif. by lipofection of EcoRI-linearized DNA into CV-1 cells (ATCC CCL-70, passage 35) by J. Lednicky (lipofection date: 1/12/97).
Classification Polyomaviridae, Polyomavirus
References

Lednicky JA, et al. Natural isolates of simian virus 40 from immunocompromised monkeys display extensive genetic heterogeneity: new implications for polyomavirus disease. J. Virol. 72: 3980-3990, 1998. PubMed: 9557685

John A Lednicky, personal communication