pUCSVH388-2 [molecular clone of Simian virus 40, SV-40] (ATCC® VRMC-10)

Organism: Simian virus 40, Simian virus 40  /  Clone Type: Clone  /  Depositors: JA Lednicky, JS Butel

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Designations pUCSVH388-2 [molecular clone of Simian virus 40, SV-40]
Species Simian virus 40, Simian virus 40
Depositors JA Lednicky, JS Butel
Isolation
Description of cloned agent:
Contains a full-length non-archetypal SV40-H388 genome cloned into pUC-19 at EcoRI site; isolated from brain tissue (animal H388). Plasmid size: 7915 bp [pUC-19 (vector): 2686 bp + SV40-H388 (insert): 5229 bp]. High-copy number; pUC-19 has a ColEI ori.
Applications
contains sequence
Vector
Construct size (kb): 7.914999961853027

DESCRIPTION OF VECTOR COMPONENT

        Type of vector:  E. coli

        Name of vector:  pUC-19

        Intact vector size (kb):  2.686 kb

Insert
DNA: genomic
Insert lengths(kb): 5.229000091552734
Tissue: monkey brain
Insert Size (kb) 5.229
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 2
Comments
Description of cloned agent:
Name: SV40
Strain: H388, variant #2
Classification: Papovavirus
Contains a full-length non-archetypal SV40-H388 genome cloned into pUC-19 at EcoRI site; isolated from brain tissue (animal H388). Plasmid size: 7915 bp [pUC-19 (vector): 2686 bp + SV40-H388 (insert): 5229 bp]. High-copy number; pUC-19 has a ColEI ori.
VRMC-10 (variant #2) is one of a matched set of 3 clones of regulatory region variants (see also VRMC-11 and VRMC-9).
Restriction enzyme analysis: BamHI--4.501,3.414; EcoRI--5.229,2.686; KpnI--6.414,1.501; SalI--7.915; SfiI--7.915; XhoI--uncut.
Sequence (viral regulatory region, T-ag carboxy terminus, and part of VP1 gene) determined by J. Lednicky. Viable clone; verif. by lipofection of EcoRI-linearized DNA into CV-1 cells (ATCC CCL-70, passage 35) by J. Lednicky (lipofection date: 1/12/97).
Classification Polyomaviridae, Polyomavirus
References

Lednicky JA, et al. Natural isolates of simian virus 40 from immunocompromised monkeys display extensive genetic heterogeneity: new implications for polyomavirus disease. J. Virol. 72: 3980-3990, 1998. PubMed: 9557685

John A Lednicky, personal communication

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