Description of cloned agent:
Strain: H388, variant #2
Contains a full-length non-archetypal SV40-H388 genome cloned into pUC-19 at EcoRI site; isolated from brain tissue (animal H388). Plasmid size: 7915 bp [pUC-19 (vector): 2686 bp + SV40-H388 (insert): 5229 bp]. High-copy number; pUC-19 has a ColEI ori.
VRMC-10 (variant #2) is one of a matched set of 3 clones of regulatory region variants (see also VRMC-11 and VRMC-9).
Restriction enzyme analysis: BamHI--4.501,3.414; EcoRI--5.229,2.686; KpnI--6.414,1.501; SalI--7.915; SfiI--7.915; XhoI--uncut.
Sequence (viral regulatory region, T-ag carboxy terminus, and part of VP1 gene) determined by J. Lednicky. Viable clone; verif. by lipofection of EcoRI-linearized DNA into CV-1 cells (ATCC CCL-70
, passage 35) by J. Lednicky (lipofection date: 1/12/97).