DNA from HSV-2 strain G [ATCC VR-734] (ATCC® VR-734D™)

Organism: Human herpesvirus 2, Herpes simplex 2  / 

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Product Format frozen

PRODUCT INFORMATION:

Total DNA from infected cells, >1 µg/mL in 1X TE buffer

100 µL per vial, shipped frozen

OD260/OD280: > 1.7

Storage Conditions -70°C or colder
Applications
It is appropriate for use in polymerase chain reaction (PCR) for viral gene products and other molecular virology applications.
Comments
This product is a preparation of viral nucleic acids, including virus genomic DNA, in total DNA isolated from Vero cells (ATCC CCL-81) infected with Human herpesvirus 2 (HSV-2) strain G. It is supplied at 100 ul per vial, shipped frozen. It is appropriate for use in polymerase chain reaction (PCR) for viral gene products and other molecular virology applications.
Biosafety Level 1
Quality Control Specifications
1. Store at -20°C or colder. -80°C or vapor-phase liquid nitrogen (-196°C) is preferred for long-term storage (more than one month).

2. Concentration is verified as > 1 µg/mL DNA by Picogreen® assay.

3. Purity is confirmed by OD260/OD280 ratio (> 1.7) of bulk DNA prior to dilution for product filling.

4. Integrity is inferred from observation of high molecular weight cellular DNA following electrophoresis 20 ul of product on a 0.8% agarose gel, visualized by ethidium bromide staining.

5. Functional activity is demonstrated by PCR amplification of a 500-3,000 bp amplicon using virus-specific primers.

6. Identity is confirmed by sequencing of ~300 base pairs of the PCR amplicon.

7. Absence of infectious virus is verified by incubation of 10% of each lot with susceptible host cells under appropriate conditions for a period of 7-14 days.
Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS