Influenza A virus (H3N2) (ATCC® VR-547)

Classification: Orthomyxoviridae, Influenzavirus A  /  Product Format: frozen 1 mL per vial

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Classification Orthomyxoviridae, Influenzavirus A
Agent Influenza A virus (H3N2)
Strain A/Aichi/2/35
Applications
Influenza Research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments Please see ATCC Virology Guide for more detailed information on propagation
Effect on Host
Hemagglutinates chicken red blood cells
Recommended Host
10 day old incubated specific pathogen free embryonated chicken eggs
Growth Conditions Temperature: 35°C
Recommendations for Infection: Dilute virus with D-PBS prior to inoculation. While candling the eggs, draw a pencil line around the air sac. Using an 18.5 gauge needle, poke a hole into the air sac. Using a 1.0 mL syringe fitted with a 22.5 gauge needle, insert the needle through the hole and into the allantoic cavity. Inject 0.2 mL of diluted inoculum into the allantoic cavity. Seal the hole with nail polish or sterile tape. Incubate eggs at 35°C 2-3 days, checking embryo viability every 12-18 hours and discard non-viable eggs. To harvest allantoic fluid, refrigerate eggs for at least 2 hour post incubation. Use sterile scissors to cut away the shell around the air sack. Aspirate the allantoic fluid with a syringe or a pipette. Avoid capturing blood or yolk in the allantoic fluid.
Incubation: 2-3 days with humidity
Effect on Host
Hemagglutinates chicken red blood cells
Name of Depositor MT Coleman
Special Collection ATCC
Source From sailor on Israeli ship docking at Aichi, Japan, 1968
References

Coleman MT, et al. The Hong Kong-68 influenza A2 variant. Lancet 2: 1384-1386, 1968. PubMed: 4177941

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation