Vaccinia virus ts mutant (ATCC® VR-3178)

Classification: Poxviridae, Orthopoxvirus  /  Product Format: frozen

Classification Poxviridae, Orthopoxvirus
Agent Vaccinia virus ts mutant
Strain IHD-W Dts93 (Dales isolate 9281)
Applications
The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U5.
Positive for Mycoplasma No
Biosafety Level 2 CDC BMBL recommends vaccination.
Isolation
derived from existing strain
Product Format frozen
Storage Conditions -70°C or colder
Comments
Selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. The non-permissive incubation temperature is 39.5oC. This mutant was not assigned to a Dales EM category. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U5.
Effect on Host
Yes, Cytopathic effect: Rounding and sloughing of infected cells
CPE: enlargement, plaques with rounding and eventual sloughing
Recommended Host

Recommended Host: BSC-40 (ATCC CRL-2761

Growth Conditions
Temperature: 31.0°C
Duration: 1-3 days; For best results, cells should be 24 to 48 hours old and 80-90% confluent at time of infection [not 100% confluent].
Effect on Host
Yes, Cytopathic effect: Rounding and sloughing of infected cells
CPE: enlargement, plaques with rounding and eventual sloughing
Name of Depositor S Dales, RC Condit, S Dales
Special Collection Orthopoxvirus
Source
derived from existing strain
References

Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807

Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746

This mutant strain was derived by Samuel Dales from the IHD-W strain of Vaccinia (ATCC VR-1441) which in turn was derived from the IHD-J strain.

cell enlargement, plaques with rounding and eventual sloughing of cells

Biosafety in Microbiological and Biomedical Laboratories 4th ed.; U.S. Department of Health and Human Services ;1999.

Non-permissive incubation temperature is 39.5C. This mutant was not assigned to a Dales EM category. It is assigned the Condit map location U5. This subtype is distinguishable from the parent IHD-J strain by its ability to elicit polykaryocytosis in all mammalian and avian cells tested and by its inability to induce an active hemagglutinin at the plasma membrane

Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570