Vaccinia virus (ATCC® VR-2153)

Classification: Poxviridae, Orthopoxvirus  /  Product Format: frozen

Permits and Restrictions

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Classification Poxviridae, Orthopoxvirus
Agent Vaccinia virus
Strain vTF7-3 [Wr]
Applications
Expresses bacteriophage T[7] RNA polymerase.
Expresses bacteriophage T[7] RNA polymerase.
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Constructed in vitro
Product Format frozen
Storage Conditions -70°C
Comments
Cells which have been infected with this construct can be transfected by plasmids which contain a gene targeted for expression inserted between a T7 promoter and T7 terminator.
Expresses bacteriophage T[7] RNA polymerase.
Recombinant virus constructed in vitro.
Expresses bacteriophage T[7] RNA polymerase.
Expression of the T7 RNA polymerase is directed by the P7.5 promoter which includes early and late regulatory signals.
Constructed by transfecting vaccinia virus-infected cells with pTF703 (ATCC 67202).
Effect on Host
Yes, in vitro effects: Cytopathic effects (plaque formation) on BS-C-1 and CV-1 monolayers
CPE, rounding, stringing, and cell destruction
Effect on Host
Yes, in vitro effects: Cytopathic effects (plaque formation) on BS-C-1 and CV-1 monolayers
CPE, rounding, stringing, and cell destruction
Name of Depositor NIH, B Moss, NIH
U.S. Patent Number
Source
Constructed in vitro
References

Fuerst TR, et al. Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc. Natl. Acad. Sci. USA 83: 8122-8126, 1986. PubMed: 3095828

Zheng Z, et al. Mutations in the carboxyl-terminal hydrophobic sequence of human cytomegalovirus glycoprotein B alter transport and protein chaperone binding. J. Virol. 70: 8029-8040, 1996. PubMed: 8892927

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation