Human echovirus 4 (ATCC® VR-1734)

Classification: Picornaviridae, Enterovirus, Human enterovirus B  /  Product Format: frozen

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Classification Picornaviridae, Enterovirus, Human enterovirus B
Agent Human echovirus 4
Strain Pesascek
Biosafety Level 2
Isolation
This item is a mycoplasma free preparation derived from ATCC VR-34 following treatment to remove mycoplasma. VR-34 will no longer be distributed.
Product Format frozen
Storage Conditions  Store at -70°C or colder
Comments
This item is a mycoplasma free preparation derived from ATCC VR-34 following treatment to remove mycoplasma. VR-34 will no longer be distributed.
Effect on Host
Cytopathic effects in tissue culture
CPE in the form of highly refractile, rounded cells throughout monolayer or in loose clusters.
Recommended Host
Production Host: LLC-Mk2 derivative cells (ATCC CCL-7.1).

Recommended Host:  LLC-Mk2 derivative cells (ATCC® CCL-7.1™).

Alternate Hosts: MkK, Mk (testis) and HED cells
Growth Conditions
Plant cells 24 hours in advance and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus diluted to provide a MOI of about 0.1. Adsorb 1 hour at 37.0°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Effect on Host
Cytopathic effects in tissue culture
CPE in the form of highly refractile, rounded cells throughout monolayer or in loose clusters.
Name of Depositor J.L. Melnick
Source
Fecal specimen from case of aseptic meningitis, Connecticut, 1951.
References

Melnick J. Application of tissue culture methods to epidemiological studies of poliomyelitis. Am. J. Public Health 44: 571-580, 1954. PubMed: 13148397

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation