Human respiratory syncytial virus, Purified (ATCC® VR-1540P)

Classification: Paramyxoviridae, Pneumovirus  /  Product Format: frozen 200 µL per vial

Classification Paramyxoviridae, Pneumovirus
Deposited As Respiratory syncytial virus
Agent Human respiratory syncytial virus, Purified
Strain A2
Applications
Respiratory research
Biosafety Level 2
Product Format frozen 200 µL per vial
Storage Conditions  -70°C or colder
Comments
VR-1540P is a purified virus product suspended in 200 µL of Hank's Balanced Salt Solution with Ca2+, Mg2+ and 25 mM HEPES Buffer. VR-1540 is prepared from original isolate deposited as VR-1302, which was grown in presence of neutralizing serum to remove contaminating adenovirus type 1.
Effect on Host
CPE, syncytial formation, rounding, and sloughing
CPE, syncytial formation, rounding, and sloughing
Recommended Host

Production Host:  HeLa (ATCC® CCL-2)

HeLa (ATCC CCL-2)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an MOI 0.1. Adsorb for 1 hour at 37°C in a humidified 5% CO2 atmosphere, rocking every 15 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 3 to 10 days
Effect on Host
CPE, syncytial formation, rounding, and sloughing
CPE, syncytial formation, rounding, and sloughing
Name of Depositor ATCC, L Potash
Source
Lower respiratory tract of infant with bronchiolitis and bronchopneumonia, Melbourne, Australia, 1961
References

Cameron R, et al. Identification of contaminating adenovirus type 1 in the ATCC reference strain of respiratory syncytial virus A2 (VR-1302). Virus Res. 92: 151-156, 2003. PubMed: 12686423

Lewis FA, et al. A syncytial virus associated with epidemic disease of the lower respiratory tract in infants and young children. Med. J. Aust. 2: 932-933, 1961.