Vaccinia virus (ATCC® VR-1508)

Classification: Poxviridae, Orthopoxvirus  /  Strain:   Modified Vaccinia Virus Ankara (MVA)  /  Product Format: frozen 1 mL per vial

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Classification Poxviridae, Orthopoxvirus
Agent Vaccinia virus
Strain Modified vaccinia virus Ankara (MVA)
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments Does not replicate in human cells or the majority of mammalian cells. This is a highly attenuated strain that some experts feel can be handled safely by trained personnel without the need for vaccination.
Effect on Host
CPE, rounding and sloughing
Recommended Host
BHK-21 (ATCC® CCL-10™)
Growth Conditions Incubation: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 3-7 days
Effect on Host
CPE, rounding and sloughing
Name of Depositor B Moss, LS Wyatt, National Institute of Allergy and Infectious Diseases
Special Collection NCRR Contract
Chain of Custody
ATCC <-- B Moss, LS Wyatt, National Institute of Allergy and Infectious Diseases <-- A Mayr
Source CVA (Ankara) strain serially passaged in chicken embryo fibroblasts and plaque purified three times

Antoine G, et al. The complete genomic sequence of the modified vaccinia Ankara strain: comparison with other orthopoxviruses. Virology 244: 365-396, 1998. PubMed: 9601507

Bernard Moss, personal communication

Mahnel H, Mayr A. Experiences with immunization against orthopox viruses of humans and animals using vaccine strain MVA. Berlin Munch. Tierarztl. Wochenschr. 107: 253-256, 1994.

Meyer H, et al. Mapping of deletions in the genome of the highly attenuated vaccinia virus MVA and their influence on virulence. J. Gen. Virol. 72: 1031-1038, 1991. PubMed: 2033387

Sutter G, Moss B. Nonreplicating vaccinia vector efficiently expresses recombinant genes. Proc. Natl. Acad. Sci. USA 89: 10847-10851, 1992. PubMed: 1438287

Cross References

Nucleotide (GenBank) : U94848 Vaccinia virus strain Ankara, complete genomic sequence

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation