Human herpesvirus 4 (ATCC® VR-1492)

Classification: Herpesviridae, Lymphocryptovirus  /  Product Format: frozen 1 mL per vial

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Classification Herpesviridae, Lymphocryptovirus
Agent Human herpesvirus 4
Strain B95-8
Common Name Epstein-Barr virus
Applications
(1984), the B95-8 cells used to produce this strain of EBV are infected with and may also shed an uncharacterized type D retrovirus.
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments
A 1 mL aliquot of this EBV preparation is capable of transforming B-lymphocytes from a preparation of normal human lymphocytes when co-cultivated with a feed layer culture (such as ATCC® 55-X™). VR-1492 is the designation for the ATCC® set of five 1 mL vials of VR-1491. VR-1491 is EBV virus shed in the supernatant of persistently infected and transformed B95-8 cells. The EBV virus itself is of human origin. The B95-8 cells used to produce this strain of EBV are infected with and may also shed an uncharacterized type D retrovirus.
Effect on Host
EBV transforms/immortalizes human and other primate B-lymphocytes. Indicators of transformed cells include cone-shaped cells, cells with spike-like projections, and small clusters of healthy cells.
Recommended Host
B95-8 cells
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Incubate in a humidified 5% CO2 atmosphere until indicators of successful transformation appear.
Incubation: 10-25 days
Effect on Host
EBV transforms/immortalizes human and other primate B-lymphocytes. Indicators of transformed cells include cone-shaped cells, cells with spike-like projections, and small clusters of healthy cells.
Name of Depositor G Miller
Special Collection NCRR Contract
Source Human lymphoblastoid cell line (883L) derived from a patient with transfusion-induced infectious mononucleosis.
References

Caputo JL, et al. An effective method for establishing Human B lymphoblastic cell lines using Epstein-Barr Virus. J. Tissue Culture Methods 13: 39-44, 1991.

Miller G, et al. Epstein-Barr virus: transformation, cytopathic changes, and viral antigens in squirrel monkey and marmoset leukocytes. Proc. Natl. Acad. Sci. USA 69: 383-387, 1972. PubMed: 4333982

Tumilowicz JJ, et al. Presence of retrovirus in the B95-8 Epstein-Barr virus-producing cell line from different sources. In Vitro 20: 486-492, 1984. PubMed: 6086497

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation