Human herpesvirus 4 (ATCC® VR-1492)

Classification: Herpesviridae, Lymphocryptovirus  /  Product Format: frozen 1 mL per vial

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Classification Herpesviridae, Lymphocryptovirus
Deposited As Epstein-Barr virus
Agent Human herpesvirus 4
Strain B95-8
Common Name Epstein-Barr virus
Applications
(1984), the B95-8 cells used to produce this strain of EBV are infected with and may also shed an uncharacterized type D retrovirus.
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments
A 1 mL aliquot of this EBV preparation is capable of transforming B-lymphocytes from a preparation of normal human lymphocytes when co-cultivated with a feed layer culture (such as ATCC® 55-X™). VR-1492 is the designation for the ATCC® set of five 1 mL vials of VR-1491. VR-1491 is EBV virus shed in the supernatant of persistently infected and transformed B95-8 cells. The EBV virus itself is of human origin. The B95-8 cells used to produce this strain of EBV are infected with and may also shed an uncharacterized type D retrovirus.
Effect on Host
EBV transforms/immortalizes human and other primate B-lymphocytes. Indicators of transformed cells include cone-shaped cells, cells with spike-like projections, and small clusters of healthy cells.
Recommended Host
B95-8 cells
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Incubate in a humidified 5% CO2 atmosphere until indicators of successful transformation appear.
Incubation: 10-25 days
Effect on Host
EBV transforms/immortalizes human and other primate B-lymphocytes. Indicators of transformed cells include cone-shaped cells, cells with spike-like projections, and small clusters of healthy cells.
Name of Depositor G Miller
Special Collection NCRR Contract
Source Human lymphoblastoid cell line (883L) derived from a patient with transfusion-induced infectious mononucleosis.
References

Caputo JL, et al. An effective method for establishing Human B lymphoblastic cell lines using Epstein-Barr Virus. J. Tissue Culture Methods 13: 39-44, 1991.

Miller G, et al. Epstein-Barr virus: transformation, cytopathic changes, and viral antigens in squirrel monkey and marmoset leukocytes. Proc. Natl. Acad. Sci. USA 69: 383-387, 1972. PubMed: 4333982

Tumilowicz JJ, et al. Presence of retrovirus in the B95-8 Epstein-Barr virus-producing cell line from different sources. In Vitro 20: 486-492, 1984. PubMed: 6086497

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation