Herpesvirus saimiri (ATCC® VR-1396)

Classification: Herpesviridae, Rhadinovirus  /  Product Format: frozen

Classification Herpesviridae, Rhadinovirus
Agent Herpesvirus saimiri
Strain C-488
Common Name Herpesvirus saimiri
Biosafety Level 2
Isolation
Blood lymphocytes of New England Regional Primate Research Ctr. squirrel monkey (Saimiri sciureus)
Product Format frozen
Storage Conditions  -70°C or colder
Comments
This item is also available as VR-1414 (herpesvirus saimiri), which does not require a USDA permit.
Herpesvirus saimiri strain C488 transforms human T lymphocytes to antigen- and mitogen-independent, but IL-2 dependent stable growth. Virus stocks are stable for months at 4C, but titer greatly reduced by freeze-thaw cycles.
For methods of propagation see: Fickenscher and Fleckenstein, 1994. in: Methods in Molecular Genetics. (ed) K. W. Adolph, 4: 345-362, 1994.
Effect on Host
Yes, in vitro effects: Cytopathic effects (foci of detached cells boardered by rounded enlarged cells)
 CPE, foci of detached cells bordered by rounded enlarged cells
Recommended Host
OMK cells (ATCC CRL-1556)

Production Host:  OMK (ATCC® CRL-1556)

Growth Conditions
Temperature: 37.0°C
Duration: 10-20 days
Effect on Host
Yes, in vitro effects: Cytopathic effects (foci of detached cells boardered by rounded enlarged cells)
 CPE, foci of detached cells bordered by rounded enlarged cells
Name of Depositor M Daniel, R Desrosiers, B Biesinger, B Fleckenstein, H Fickenscher
Special Collection NCRR Contract
Source
Blood lymphocytes of New England Regional Primate Research Ctr. squirrel monkey (Saimiri sciureus)
References

Biesinger B, et al. The divergence between two oncogenic Herpesvirus saimiri strains in a genomic region related to the transforming phenotype. Virology 176: 505-514, 1990. PubMed: 2161148

Desrosiers RC, Falk LA. Herpesvirus saimiri strain variability. J. Virol. 43: 352-356, 1982. PubMed: 6287011

Biesinger B, et al. Stable growth transformation of human T lymphocytes by herpesvirus saimiri. Proc. Natl. Acad. Sci. USA 89: 3116-3119, 1992. PubMed: 1313581

. Methods in Molecular Genetics, Part A. : K.W. Adolph; 1993.