RAW 309 Cr.1 (ATCC® TIB-69)

Organism: Mus musculus, mouse  /  Cell Type: monocyte/macrophage  / 

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Organism Mus musculus, mouse
Cell Type monocyte/macrophage
Product Format frozen
Culture Properties adherent
Biosafety Level 2
Applications
was derived from the same tumor as RAW 309F.1.1 (see ATCC TIB-51).
The cells are negative for surface immunoglobulin (sIg-) Thy-1.2 (Thy-1.2-) and Ia (Ia-).
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor phase
Derivation
RAW 309 Cr.1 was derived from the same tumor as RAW 309F.1.1 (see ATCC TIB-51).
Antigen Expression
H-2d; H-2b
Genes Expressed
Abelson murine leukemia virus (infectious); lysozyme,H-2d; H-2b
Cellular Products
Abelson murine leukemia virus (infectious); lysozyme
Comments
RAW 309 Cr.1 was derived from the same tumor as RAW 309F.1.1 (see ATCC TIB-51).
The cells are negative for surface immunoglobulin (sIg-) Thy-1.2 (Thy-1.2-) and Ia (Ia-).
The cells will pinocytose neutral red and phagocytose latex beads and zymosan.
They will also lyse antibody coated sheep erythrocytes.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Name of Depositor WC Raschke
References

Raschke WC, et al. Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198

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These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Raschke WC, et al. Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198