WR19L (ATCC® TIB-52)

Organism: Mus musculus, mouse  /  Disease: lymphoma

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Organism Mus musculus, mouse
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2
Disease lymphoma
Strain BALB/c
Applications
The line was derived from the WR19 tumor induced by Abelson murine leukemia virus (see also ATCC TIB-70) but does not produce Abelson virus.
The cells are negative for surface immunoglobulin (sIg-) and Ia (Ia-).
The line does not pinocytose neutral red, phagocytose zymosan or latex beads and does not secrete lysozyme.
Tested and found negative for ectromelia virus (mousepox).
Derivation
The line was derived from the WR19 tumor induced by Abelson murine leukemia virus (see also ATCC TIB-70) but does not produce Abelson virus.
Antigen Expression
H-2d; Thy-1.2
Genes Expressed
Moloney murine leukemia virus,H-2d; Thy-1.2
Cellular Products
Moloney murine leukemia virus
Comments
The line was derived from the WR19 tumor induced by Abelson murine leukemia virus (see also ATCC TIB-70) but does not produce Abelson virus.
The cells are negative for surface immunoglobulin (sIg-) and Ia (Ia-).
The line does not pinocytose neutral red, phagocytose zymosan or latex beads and does not secrete lysozyme.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Name of Depositor WC Raschke
References

Raschke WC, et al. Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198

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Basic Documentation
References

Raschke WC, et al. Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198