RAW 309F.1.1 (ATCC® TIB-51)

Organism: Mus musculus, mouse  /  Disease: lymphoma

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Organism Mus musculus, mouse
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2
Disease lymphoma
Applications
The cells are negative for surface immunoglobulin (sIg-), Thy-1.2 (Thy-1.2-) and Ia (Ia-).
The cells do not pinocytose neutral red, phagocytose zymosan or latex beads and do not secrete lysozyme.
The line was derived from the RAW 309 tumor (see also ATCC TIB-69).
Tested and found negative for ectromelia virus (mousepox).
Derivation
The line was derived from the RAW 309 tumor (see also ATCC TIB-69).
Antigen Expression
H-2d; H-2b
Genes Expressed
Abelson murine leukemia virus (infectious),H-2d; H-2b
Cellular Products
Abelson murine leukemia virus (infectious)
Comments
The cells are negative for surface immunoglobulin (sIg-), Thy-1.2 (Thy-1.2-) and Ia (Ia-).
The cells do not pinocytose neutral red, phagocytose zymosan or latex beads and do not secrete lysozyme.
The line was derived from the RAW 309 tumor (see also ATCC TIB-69).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Name of Depositor WC Raschke
References

Raschke WC, et al. Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198

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These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Raschke WC, et al. Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198