HuT 102 (ATCC® TIB-162)

Organism: Homo sapiens, human  /  Cell Type: cutaneous T lymphocyte  /  Tissue: peripheral blood  /  Disease: lymphoma; mycosis fungoides

Organism Homo sapiens, human
Tissue peripheral blood
Cell Type cutaneous T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension, multicell aggregates
Biosafety Level 2 [These cells release a type C retrovirus that has been associated with human T cell lymphomas and should be handled as a biohazard.]
Disease lymphoma; mycosis fungoides
Age 26 years
Gender male
Ethnicity Black
Storage Conditions liquid nitrogen vapor phase
Images
Derivation
The Hut 102 cell line was derived from the peripheral blood of a 26 year old Black male patient with mycosis fungoides.
Clinical Data
male
The Hut 102 cell line was derived from the peripheral blood of a 26 year old Black male patient with mycosis fungoides.
26 years
Black
Antigen Expression
Antigen expression: CD4; Homo sapiens
Receptor Expression
Receptor expression: interleukin 2 (IL-2)
Genes Expressed
HTLV-I (human T cell leukemia virus)
Comments This cell line has the properties of a mature human T cell with helper/inducer activity.
It has receptors for IL-2, and the growth rate is stimulated by IL-2.
This cell line is E-rosette positive (E+), and negative for Epstein-Barr virus nuclear antigen (EBNA-), surface immunoglobulin (sIg-) and terminal deoxynucleotidyl transferase (TdT-).
The cell line was originally thought to produce IL-2; however, recent studies have failed to detect IL-2 mRNA production in Hut 102 cells.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 x 105 viable cells/mL. Maintain cell density between 2 x 105 and 8 x 105 viable cells/mL. Do not allow density to exceed 1 x 106 cells/mL. Background debris is often present.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 8,11
D13S317: 11,13
D16S539: 12
D5S818: 8,13
D7S820: 8,10
THO1: 7,8
TPOX: 6
vWA: 16,19
Population Doubling Time about 41 hrs
Name of Depositor AF Gazdar
References

Gootenberg JE, et al. Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor. J. Exp. Med. 154: 1403-1418, 1981. PubMed: 6975346

Gazdar AF, et al. Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas. Blood 55: 409-417, 1980. PubMed: 6244013

Hsu SM, et al. Expression of p55 (Tac) interleukin-2 receptor (IL-2R), but not p75 IL- 2R, in cultured H-RS cells and H-RS cells in tissues. Am. J. Pathol. 136: 735-744, 1990. PubMed: 1691591

Poiesz BJ, et al. Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma. Proc. Natl. Acad. Sci. USA 77: 7415-7419, 1980. PubMed: 6261256

Wano Y, et al. Stable expression of the tax gene of type I human T-cell leukemia virus in human T cells activates specific cellular genes involved in growth. Proc. Natl. Acad. Sci. USA 85: 9733-9737, 1988. PubMed: 3059351

Basic Documentation
Other Documentation
References

Gootenberg JE, et al. Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor. J. Exp. Med. 154: 1403-1418, 1981. PubMed: 6975346

Gazdar AF, et al. Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas. Blood 55: 409-417, 1980. PubMed: 6244013

Hsu SM, et al. Expression of p55 (Tac) interleukin-2 receptor (IL-2R), but not p75 IL- 2R, in cultured H-RS cells and H-RS cells in tissues. Am. J. Pathol. 136: 735-744, 1990. PubMed: 1691591

Poiesz BJ, et al. Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma. Proc. Natl. Acad. Sci. USA 77: 7415-7419, 1980. PubMed: 6261256

Wano Y, et al. Stable expression of the tax gene of type I human T-cell leukemia virus in human T cells activates specific cellular genes involved in growth. Proc. Natl. Acad. Sci. USA 85: 9733-9737, 1988. PubMed: 3059351