HuT 102 (ATCC® TIB-162)

Organism: Homo sapiens, human  /  Cell Type: cutaneous T lymphocyte  /  Tissue: peripheral blood  /  Disease: lymphoma; mycosis fungoides

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Organism Homo sapiens, human
Tissue peripheral blood
Cell Type cutaneous T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension, multicell aggregates
Biosafety Level 2 [These cells release a type C retrovirus that has been associated with human T cell lymphomas and should be handled as a biohazard.]

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease lymphoma; mycosis fungoides
Age 26 years
Gender male
Ethnicity Black
Storage Conditions liquid nitrogen vapor phase
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Derivation
The Hut 102 cell line was derived from the peripheral blood of a 26 year old Black male patient with mycosis fungoides.
Clinical Data
male
The Hut 102 cell line was derived from the peripheral blood of a 26 year old Black male patient with mycosis fungoides.
26 years
Black
Antigen Expression
Antigen expression: CD4; Homo sapiens
Receptor Expression
Receptor expression: interleukin 2 (IL-2)
Genes Expressed
HTLV-I (human T cell leukemia virus)
Comments This cell line has the properties of a mature human T cell with helper/inducer activity.
It has receptors for IL-2, and the growth rate is stimulated by IL-2.
This cell line is E-rosette positive (E+), and negative for Epstein-Barr virus nuclear antigen (EBNA-), surface immunoglobulin (sIg-) and terminal deoxynucleotidyl transferase (TdT-).
The cell line was originally thought to produce IL-2; however, recent studies have failed to detect IL-2 mRNA production in Hut 102 cells.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 x 105 viable cells/mL. Maintain cell density between 2 x 105 and 8 x 105 viable cells/mL. Do not allow density to exceed 1 x 106 cells/mL. Background debris is often present.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 8,11
D13S317: 11,13
D16S539: 12
D5S818: 8,13
D7S820: 8,10
THO1: 7,8
TPOX: 6
vWA: 16,19
Population Doubling Time about 41 hrs
Name of Depositor AF Gazdar
References

Gootenberg JE, et al. Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor. J. Exp. Med. 154: 1403-1418, 1981. PubMed: 6975346

Gazdar AF, et al. Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas. Blood 55: 409-417, 1980. PubMed: 6244013

Hsu SM, et al. Expression of p55 (Tac) interleukin-2 receptor (IL-2R), but not p75 IL- 2R, in cultured H-RS cells and H-RS cells in tissues. Am. J. Pathol. 136: 735-744, 1990. PubMed: 1691591

Poiesz BJ, et al. Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma. Proc. Natl. Acad. Sci. USA 77: 7415-7419, 1980. PubMed: 6261256

Wano Y, et al. Stable expression of the tax gene of type I human T-cell leukemia virus in human T cells activates specific cellular genes involved in growth. Proc. Natl. Acad. Sci. USA 85: 9733-9737, 1988. PubMed: 3059351

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Gootenberg JE, et al. Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor. J. Exp. Med. 154: 1403-1418, 1981. PubMed: 6975346

Gazdar AF, et al. Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas. Blood 55: 409-417, 1980. PubMed: 6244013

Hsu SM, et al. Expression of p55 (Tac) interleukin-2 receptor (IL-2R), but not p75 IL- 2R, in cultured H-RS cells and H-RS cells in tissues. Am. J. Pathol. 136: 735-744, 1990. PubMed: 1691591

Poiesz BJ, et al. Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma. Proc. Natl. Acad. Sci. USA 77: 7415-7419, 1980. PubMed: 6261256

Wano Y, et al. Stable expression of the tax gene of type I human T-cell leukemia virus in human T cells activates specific cellular genes involved in growth. Proc. Natl. Acad. Sci. USA 85: 9733-9737, 1988. PubMed: 3059351