Saccharomyces cerevisiae Meyen ex E.C. Hansen (ATCC® MYA-4902)

Alternate State: Candida robusta Diddens et Lodder  /  Strain Designations: H4S47C  /  Product Format: frozen

Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
Strain Designations H4S47C
Alternate State Candida robusta Diddens et Lodder
Biosafety Level 1
Product Format frozen
Type Strain no
Genotype MATa, his3delta1, leu2delta0, met15delta0, URA3
Preceptrol® no
Mating Type a
Comments
Temperature sensitive
HHF1 modfied to H4S47C via site directed mutagenesis. The second histone H4 gene, HHF2, was knocked out and replaced with the URA3 selection marker.
Morphology On YEPD medium at 25°C after 5 days cells are globose, ovoidal or elongate and are usually isolated or in small groups. Growth is butyrous and light cream colored.
Medium Medium 1069: YPAD medium
Medium 1245: YEPD
Medium 200: YM agar or YM broth
Growth Conditions
Temperature: 20°C to 25°C
Atmosphere: Typical aerobic
Morphology On YEPD medium at 25°C after 5 days cells are globose, ovoidal or elongate and are usually isolated or in small groups. Growth is butyrous and light cream colored.
Name of Depositor K Brogaard, J Widom
Special Collection ATCC
Chain of Custody
ATCC <-- K Brogaard, J Widom
Isolation
Targeted mutagenesis from BY4741.
References

Brogaard K, et al. A map of nucleosome positions in yeast at base-pair resolution. Nature 486(7404): 496-501, 2012. PubMed: 22722846