pGRN145 plasmid in E. coli GC10 (ATCC® MBA-141)

Depositors: Geron Corporation

Permits and Restrictions

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Designations pGRN145 plasmid in E. coli GC10
Depositors Geron Corporation
Applications Expression vector [telomerase reverse transcriptase] (EST2; TCS1; TP2; TRT; hEST2; telomerase catalytic subunit)
Vector
Total size (kb): 14.002
Bacterial selection marker: cmlR

 

Features:
hTERT: 4225-7623
HygB: 9216-10238 (complementary)
CatR: 10822-11480
AmpS: 12941-13799 (complementary)
PAC: 368-964 (complementary)
F1 ori: 11501-11685 (complementary)
MPSV: 3643-4119 (promoter)
Ori: 12165-12747

 

Restriction digests of this clone gave the following sizes (in kb): HindIII/KpnI - 10.5, 3.5; EcoRI - 10.6, 3.4; XhoI - 12.0, 2.0.

Insert
Gene: Telomerase reverse transcriptase (TERT)
Source: Human
Insert size (kb): 3.398
GenBank accession: NM_003219
Media ATCC® Medium 2585: LB Medium (ATCC medium 1065) with 37 mcg/ml chloramphenicol
Biosafety Level 1
Shipping Information Frozen glycerol stock of E. coli GC10 containing the plasmid
Restrictions

This material is subject to claims under U.S. Patent Nos. 6,261,836 and 6,337,200, other pending patent applications, and foreign counterparts thereof. It is required that the Addendum for Noncommercial and Academic Organizations be signed and returned to ATCC before shipment.  Commercial and For Profit Organizations need to obtain a license from Geron Corporation by contacting info@geron.com before shipment.

Product Sheet
Product Sheet
Product Sheet
Attachments MSDS
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