Calu-1 (ATCC® HTB-54)

Organism: Homo sapiens, human  /  Tissue: lung; derived from metastatic site: pleura  /  Disease: grade III,epidermoid carcinoma

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Organism Homo sapiens, human
Tissue lung; derived from metastatic site: pleura
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease grade III,epidermoid carcinoma
Age 47 years
Gender male
Ethnicity Caucasian
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype The stemline chromosome number is hypotriploid and the 2S component occurred at 14.2%. Modal chromosome number is 62. Seven markers occurred frequently, M1 (two copies per cell), M6 and M7 were found in most cells; M2 and M3, and M4 and M5 appeared to be mutually exclusive, i.e., only one of M2 or M3, and one of M4 or M5 were present in each cell. A Y chromosome was not detected by QM band examination, although the cell line was initiated from a male.
Clinical Data
47 years
Caucasian
male
Antigen Expression
Antigen expression: Blood Type A; Rh+; HLA A10, A11, B15, Bw35
Tumorigenic Yes
Effects
Yes, in nude mice; forms epidermoid carcinomas
Yes, in steroid treated hamsters
Comments
Ultrastructural features include numerous microvilli, prominent RER, lysosomes, lipid inclusions, no virus particles.
Contains the ras (K-ras) oncogene.
Complete Growth Medium The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Interval: every 6 to 8 days
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 11,12
D16S539: 11
D5S818: 10,12
D7S820: 9,10
THO1: 9,9.3
TPOX: 8
vWA: 15,16
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 1-2
PGM1, 1-2
PGM3, 1
Name of Depositor J Fogh
References

Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975.

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034

Tew KD, Taylor DM. Studies with cyclophosphamide labeled with phosphorus-32: nucleic acid alkylation and its effect on DNA synthesis in rat tumor and normal tissues. J. Natl. Cancer Inst. 58: 1413-1418, 1977. PubMed: 857031

Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the cells subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with the Office of Technology Development, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065. Contact email: otd@mskcc.org

References

Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975.

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034

Tew KD, Taylor DM. Studies with cyclophosphamide labeled with phosphorus-32: nucleic acid alkylation and its effect on DNA synthesis in rat tumor and normal tissues. J. Natl. Cancer Inst. 58: 1413-1418, 1977. PubMed: 857031

Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047