NCI-H676B [H676B] ATCC ® HTB-179™ Homo sapiens adenocarcino

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism	Homo sapiens, human
Product Format	frozen
Morphology	epithelial
Culture Properties	suspension, multicell aggregates (gland-like clusters in suspension; the viability of cells in clusters is better than the viability of the single cells in the cultures)
Biosafety Level	1
Disease	adenocarcinoma
Age	63 years
Gender	male
Ethnicity	Caucasian
Applications	The NCI-H676B cell line was derived by A.F. Gazdar and H. Oie in 1984 from the pleural fluid of a patient with adenocarcinoma of the lung taken prior to treatment. The cells produce a abnormal size p53 mRNA (2.3 kb) as well as the normal size mRNA (2.8 kb). The line does not exhibit any gross structural DNA abnormalities. The cells produce mucin, and occasionally signet ring cells are formed.
Storage Conditions	liquid nitrogen vapor phase

Karyotype	This is a hypodiploid cell line; modal number = 43; range = 39 to 47. Marker chromosomes consisted of over 70% of the chromosomes in each cell complement. Among the identifiable markers were t(1q8q), der(3)t(3;?)(q29;?), t(2q7q), der(9)t(9;14)(p13;q11) and der(11)t(?q23;?). All markers had a single copy per cells. The N5, N9, N10, N17, N18, N20, N21 and the X were the only structurally normal chromosomes found. Except for the paired N5, all others had single copies per cell.
Derivation	The NCI-H676B cell line was derived by A.F. Gazdar and H. Oie in 1984 from the pleural fluid of a patient with adenocarcinoma of the lung taken prior to treatment.
Clinical Data	The NCI-H676B cell line was derived by A.F. Gazdar and H. Oie in 1984 from the pleural fluid of a patient with adenocarcinoma of the lung taken prior to treatment. male Caucasian 63 years
Genes Expressed	mucin
Comments	The NCI-H676B cell line was derived by A.F. Gazdar and H. Oie in 1984 from the pleural fluid of a patient with adenocarcinoma of the lung taken prior to treatment. The cells produce a abnormal size p53 mRNA (2.3 kb) as well as the normal size mRNA (2.8 kb). The line does not exhibit any gross structural DNA abnormalities. The cells produce mucin, and occasionally signet ring cells are formed.

Complete Growth Medium	ACL-4 medium (serum-free) The base medium for this cell line is ATCC formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium: 0.02 mg/ml insulin 0.01 mg/ml transferrin 25 nM sodium selenite (final conc.) 50 nM Hydrocortisone (final conc.) 1 ng/ml Epidermal Growth Factor (do not filter) 0.01 mM ethanolamine (final conc.) 0.01 mM phosphorylethanolamine (final conc.) 100 pM triiodothyronine (final conc.) 0.5% (w/v) bovine serum albumin (final conc.) 0.5 mM sodium pyruvate (final conc.) extra 2mM L-glutamine (for final conc. of 4.5mM)
Subculturing	Protocol: This line grows as aggregates of cells in suspension. Culture can be maintained by addition of medium or by replacement of medium. Alternatively, the cells may be collected by centrifugation and dispersed into fresh medium. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended Medium Renewal: 2 to 3 times per week
Cryopreservation	Freeze medium: culture medium, 82.5%; fetal bovine serum, 10%; DMSO, 7.5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5%

STR Profile	Amelogenin: X CSF1PO: 9 D13S317: 10 D16S539: 11,12 D5S818: 11 D7S820: 12 THO1: 9.3 TPOX: 8,11 vWA: 16,19
Isoenzymes	AK-1, 2 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 0 PGM1, 1-2 PGM3, 2

Name of Depositor	AF Gazdar, JD Minna
Year of Origin	1984
References	Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494 gland-like clusters in suspension; the viability of cells in clusters is better than the viability of the single cells in the cultures gland-like clusters in suspension; the viability of cells in clusters is better than the viability of the single cells in the cultures

Name of Depositor

AF Gazdar, JD Minna

Year of Origin

1984

References

Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494

gland-like clusters in suspension; the viability of cells in clusters is better than the viability of the single cells in the cultures

Permits

These permits may be required for shipping this product:

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

Basic Documentation

References

Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494

gland-like clusters in suspension; the viability of cells in clusters is better than the viability of the single cells in the cultures

NCI-H676B [H676B] (ATCC® HTB-179™)