MECA-367 (ATCC® HB-9478)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody specifically blocks lymphocyte adhesion to mouse Peyer's patch endothelia and does not block lymphocyte binding to peripheral lymph nodes.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse Peyer's patch endothelial cells (MAdCAM-1)
Cellular Products
immunoglobulin; monoclonal antibody; against mouse Peyer's patch endothelial cells (MAdCAM-1)
Comments
Animals were immunized with mouse lymphnode stroma.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody specifically blocks lymphocyte adhesion to mouse Peyer's patch endothelia and does not block lymphocyte binding to peripheral lymph nodes.
It recognizes the N terminal domain of mouse mucosal vascular addressin (MAdCAM-1) involved in alpha 4, beta 7 (a4b7) integrin binding.
Complete Growth Medium HB101 medium, 100% - OR - RPMI 1640 medium, 90%; fetal bovine serum, 10%
Complete Growth Medium
Culture Medium: RPMI 1640 medium, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Culture Conditions
Temperature: 37.0°C
Isotype IgG2a
Name of Depositor Stanford University Board of Trustees
References

Butcher EC. Method to control leukocyte extravasation. US Patent 5,403,919 dated Apr 4 1995

Streeter PR, et al. A tissue-specific endothelial cell molecule involved in lymphocyte homing. Nature 331: 41-46, 1988. PubMed: 3340147

Berlin C, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74: 185-195, 1993. PubMed: 7687523

Briskin MJ, et al. MAdCAM-1 has homology to immunoglobulin and mucin-like adhesion receptors and to IgA1. Nature 363: 461-464, 1993. PubMed: 8502297

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Butcher EC. Method to control leukocyte extravasation. US Patent 5,403,919 dated Apr 4 1995

Streeter PR, et al. A tissue-specific endothelial cell molecule involved in lymphocyte homing. Nature 331: 41-46, 1988. PubMed: 3340147

Berlin C, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74: 185-195, 1993. PubMed: 7687523

Briskin MJ, et al. MAdCAM-1 has homology to immunoglobulin and mucin-like adhesion receptors and to IgA1. Nature 363: 461-464, 1993. PubMed: 8502297