M 111 [M111] (ATCC® HB-8438)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with a cell surface antigen on human melanoma cells (gp110).
The antibody reacts with the M-4 antigen system.
Genes Expressed
immunoglobulin; monoclonal antibody; against human cutaneous melanocytes (M-4 antigen system)
Cellular Products
immunoglobulin; monoclonal antibody; against human cutaneous melanocytes (M-4 antigen system)
Comments
Animals were immunized with the human cutaneous melanoma cells.
The antibody reacts with a cell surface antigen on human melanoma cells (gp110).
The antibody reacts with the M-4 antigen system.
Complete Growth Medium RPMI 1640 medium with non-essential amino acids, 90%; fetal bovine serum, 10%
Complete Growth Medium
Culture Medium: RPMI 1640 with non-essential amino acids, 90%; fetal bovine
serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium, 90%; DMSO, 10%
Isotype IgG1
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.

References

Houghton AN, et al. Surface antigens of melanocytes and melanomas. Markers of melanocyte differentiation and melanoma subsets. J. Exp. Med. 156: 1755-1766, 1982. PubMed: 7175440

Real FX, et al. Surface antigens of melanomas and melanocytes defined by mouse monoclonal antibodies: Specificity analysis and comparison of antigen expression in cultured cells and tissues. Cancer Res. 45: 4401-4411, 1985. PubMed: 4028024

Cairncross JG, et al. Monoclonal antibodies against melanocytes. US Patent 4,806,628 dated Feb 21 1989

Oettgen HF, et al. Monoclonal antibodies to cell surface antigens of human malignant melanoma. US Patent 4,808,704 dated Feb 28 1989