26-8-16S (ATCC® HB-42)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against I-A k; cytotoxic
Cellular Products
immunoglobulin; monoclonal antibody; against I-A k; cytotoxic
Comments
Animals were immunized with spleen cells from A.TL mice.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
  • Complete Growth Medium
    Culture Medium: Modified Dulbecco's medium (see below), 90%; fetal bovine
    serum, 10%.
    DME w/HEPES (10mM) 100 mL
    L-Glutamine (100x) 1 mL
    Solution I 1 mL
    Non-essential amino acids (100x) 1 mL
    NCTC 135 10 mL
    Fetal Bovine Serum 12 mL
    Solution I (100x)
    1. 1320 mg oxalacetic acid (100 mM, MW 132).
    2. 80 mg crystalline bovine insulin (20 units/mL, 25 units/mg).
    Add 1 and 2, stir at 37°C. Add Na pyruvate 550 mg (50 mM, FW 110). Bring up to
    100 mL with distilled water. Stir at 37°C until solution dissolves. Filter,
    aliquot and store frozen.
    Subculturing
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
    Isotype IgM
    Name of Depositor DH Sachs, K Ozato