16-10A1 (ATCC® HB-301)

Organism: hamster (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

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Organism hamster (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with and blocks the costimulatory activity of CD80.
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against murine CD80 (B7-1)
Cellular Products
immunoglobulin; monoclonal antibody; against murine CD80 (B7-1)
Comments
Animals were immunized with CD80 transfected CHO cells.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with and blocks the costimulatory activity of CD80.
It does not affect the activity of CD86 (B7-2).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype IgG
Name of Depositor H Reiser, BW Janicki
Deposited As hamster (B cell); mouse (myeloma)
References

Razi-Wolf Z, et al. Expression and function of the costimulatory molecule B7 on murine Langerhans cells: evidence for an alternative CTLA-4 ligand. Eur. J. Immunol. 24: 805-811, 1994. PubMed: 7512030

Razi-Wolf Z, et al. Expression and function of the murine B7 antigen, the major costimulatory molecule expressed by peritoneal exudate cells. Proc. Natl. Acad. Sci. USA 89: 4210-4214, 1992. PubMed: 1373896

Razi-Wolf Z, et al. Evidence for an additional ligand, distinct from B7, for the CTLA-4 receptor. Proc. Natl. Acad. Sci. USA 90: 11182-11186, 1993. PubMed: 7504299

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Razi-Wolf Z, et al. Expression and function of the costimulatory molecule B7 on murine Langerhans cells: evidence for an alternative CTLA-4 ligand. Eur. J. Immunol. 24: 805-811, 1994. PubMed: 7512030

Razi-Wolf Z, et al. Expression and function of the murine B7 antigen, the major costimulatory molecule expressed by peritoneal exudate cells. Proc. Natl. Acad. Sci. USA 89: 4210-4214, 1992. PubMed: 1373896

Razi-Wolf Z, et al. Evidence for an additional ligand, distinct from B7, for the CTLA-4 receptor. Proc. Natl. Acad. Sci. USA 90: 11182-11186, 1993. PubMed: 7504299