BBM.1 (ATCC® HB-28)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human Beta-2 microglobulin; cytotoxic
Cellular Products
immunoglobulin; monoclonal antibody; against human Beta-2 microglobulin; cytotoxic
Comments
Animals were immunized with MOLT-4 cells (ATCC CRL-1582, a human T cell line).
Spleen cells were fused with P3X63Ag8 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG2b
Antigenic Determinant Microglobulin, beta-2, human
Name of Depositor P Parham
References

Brodsky FM, et al. Characterization of a monoclonal anti-B2-microglobulin antibody and its use in the genetic and biochemical analysis of major histocompatibility antigens. Eur. J. Immunol. 9: 536-545, 1979. PubMed: 91522

Parham P, et al. Arginine 45 is a major part of the antigenic determinant of human beta2-microglobulin recognized by mouse monoclonal antibody BBM.1. J. Biol. Chem. 258: 6179-6186, 1983. PubMed: 6189821

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Brodsky FM, et al. Characterization of a monoclonal anti-B2-microglobulin antibody and its use in the genetic and biochemical analysis of major histocompatibility antigens. Eur. J. Immunol. 9: 536-545, 1979. PubMed: 91522

Parham P, et al. Arginine 45 is a major part of the antigenic determinant of human beta2-microglobulin recognized by mouse monoclonal antibody BBM.1. J. Biol. Chem. 258: 6179-6186, 1983. PubMed: 6189821