CC15 (ATCC® HB-265)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is specific for the bovine WC1 glycoprotein.
The antibody reacts with and precipitates both the 205000 and the 215000 dalton WC1 molecules and and addtional 300000 dalton glycoprotein.
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with NS1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against bovine WC1 T cell antigen (BoWC1)
Cellular Products
immunoglobulin; monoclonal antibody; against bovine WC1 T cell antigen (BoWC1)
Comments
Animals were immunized with bovine lymphocytes.
Spleen cells were fused with NS1 myeloma cells.
The antibody is specific for the bovine WC1 glycoprotein.
The antibody reacts with and precipitates both the 205000 and the 215000 dalton WC1 molecules and and addtional 300000 dalton glycoprotein.
This antibody was included in the panel of the International Workshop on Bovine, Sheep and Goat Leukocyte Differentiation Antigens in 1989 and 1992.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(5) cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype mouse IgG2a
Name of Depositor CJ Howard
Deposited As mouse (B cell); mouse (myeloma)
References

Carr MM, et al. Expression on porcine gamma delta lymphocytes of a phylogenetically conserved surface antigen previously restricted in expression to ruminant gamma delta T lymphocytes. Immunology 81: 36-40, 1994. PubMed: 8132217

. . Rev. Sci. Tech. Off. Int. Epizoot. 9: 95-103, 1990.

Howard CJ, et al. In vivo depletion of BoT4 (CD4) and of non-T4/T8 lymphocyte subsets in cattle with monoclonal antibodies. Eur. J. Immunol. 19: 757-764, 1989. PubMed: 2567247

Clevers H, et al. Identification of a bovine surface antigen uniquely expressed on CD4- CD8- T cell receptor gamma/delta+ T lymphocytes. Eur. J. Immunol. 20: 809-817, 1990. PubMed: 1971793

Oldham G, Howard CJ. Suppression of bovine lymphocyte responses to mitogens following in vivo and in vitro treatment with dexamethasone. Vet. Immunol. Immunopathol. 30: 161-177, 1992. PubMed: 1534431

Howard CJ, et al. Immunity to bovine virus diarrhoea virus in calves: the role of different T-cell subpopulations analysed by specific depletion in vivo with monoclonal antibodies. Vet. Immunol. Immunopathol. 32: 303-314, 1992. PubMed: 1321529

Howard CJ, et al. Distinction of naive and memory BoCD4 lymphocytes in calves with a monoclonal antibody, CC76, to a restricted determinant of the bovine leukocyte-common antigen, CD45. Eur. J. Immunol. 21: 2219-2226, 1991. PubMed: 1716214

Oldham G, et al. In vivo role of lymphocyte subpopulations in the control of virus excretion and mucosal antibody responses of cattle infected with rotavirus. J. Virol. 67: 5012-5019, 1993. PubMed: 8392626

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Carr MM, et al. Expression on porcine gamma delta lymphocytes of a phylogenetically conserved surface antigen previously restricted in expression to ruminant gamma delta T lymphocytes. Immunology 81: 36-40, 1994. PubMed: 8132217

. . Rev. Sci. Tech. Off. Int. Epizoot. 9: 95-103, 1990.

Howard CJ, et al. In vivo depletion of BoT4 (CD4) and of non-T4/T8 lymphocyte subsets in cattle with monoclonal antibodies. Eur. J. Immunol. 19: 757-764, 1989. PubMed: 2567247

Clevers H, et al. Identification of a bovine surface antigen uniquely expressed on CD4- CD8- T cell receptor gamma/delta+ T lymphocytes. Eur. J. Immunol. 20: 809-817, 1990. PubMed: 1971793

Oldham G, Howard CJ. Suppression of bovine lymphocyte responses to mitogens following in vivo and in vitro treatment with dexamethasone. Vet. Immunol. Immunopathol. 30: 161-177, 1992. PubMed: 1534431

Howard CJ, et al. Immunity to bovine virus diarrhoea virus in calves: the role of different T-cell subpopulations analysed by specific depletion in vivo with monoclonal antibodies. Vet. Immunol. Immunopathol. 32: 303-314, 1992. PubMed: 1321529

Howard CJ, et al. Distinction of naive and memory BoCD4 lymphocytes in calves with a monoclonal antibody, CC76, to a restricted determinant of the bovine leukocyte-common antigen, CD45. Eur. J. Immunol. 21: 2219-2226, 1991. PubMed: 1716214

Oldham G, et al. In vivo role of lymphocyte subpopulations in the control of virus excretion and mucosal antibody responses of cattle infected with rotavirus. J. Virol. 67: 5012-5019, 1993. PubMed: 8392626