MD2 (ATCC® HB-229)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
In the mouse thymus, MD2 is specific for dendritic cells in the medulla.
The antibody cross-reacts with rare cells in the human thymic medulla and faintly with Hassall's bodies.
It also reacts with epithelial elements in a variety of mouse tissues.
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse medullary thymic epithelium
Cellular Products
immunoglobulin; monoclonal antibody; against mouse medullary thymic epithelium
Comments
Animals were immunized with mouse thymic stromal tissue.
Spleen cells were fused with Sp2/0 myeloma cells.
In the mouse thymus, MD2 is specific for dendritic cells in the medulla.
The antibody cross-reacts with rare cells in the human thymic medulla and faintly with Hassall's bodies.
It also reacts with epithelial elements in a variety of mouse tissues.
Complete Growth Medium RPMI 1640 medium with non-essential amino acids, 1 mM sodium pyruvate and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Complete Growth Medium
Culture Medium: RPMI medium 1640 with non-essential amino acids, 1 mM sodium
pyruvate and 50 uM 2-mercaptoethanol, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgM
Name of Depositor RV Rouse
References

Rouse RV, et al. Monoclonal antibodies reactive with subsets of mouse and human thymic epithelial cells. J. Histochem. Cytochem. 36: 1511-1517, 1988. PubMed: 2461413

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Rouse RV, et al. Monoclonal antibodies reactive with subsets of mouse and human thymic epithelial cells. J. Histochem. Cytochem. 36: 1511-1517, 1988. PubMed: 2461413