H57-597 (ATCC® HB-218)

Organism: Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with all alpha,beta TCR bearing murine T cells, but does not react with receptors on gamma,delta positive T cells.
The antibody can directly activate alpha,beta TCR bearing T cells and can be used to immunoprecipitate the receptor from T cell lysates.
Derivation
Animals were immunized with affinity-purified T cell antigen receptor (TCR) from the murine T cell hybridoma DO-11.10.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse T cell antigen receptor
Cellular Products
immunoglobulin; monoclonal antibody; against mouse T cell antigen receptor
Comments
Animals were immunized with affinity-purified T cell antigen receptor (TCR) from the murine T cell hybridoma DO-11.10.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with all alpha,beta TCR bearing murine T cells, but does not react with receptors on gamma,delta positive T cells.
The antibody can directly activate alpha,beta TCR bearing T cells and can be used to immunoprecipitate the receptor from T cell lysates.
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere
  • 0.05 mM 2-Mercaptoethanol

Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG
Name of Depositor RT Kubo
References

Kubo RT, et al. Characterization of a monoclonal antibody which detects all murine alphabeta T cell receptors. J. Immunol. 142: 2736-2742, 1989. PubMed: 2467936

White J, et al. The V beta-specific superantigen staphylococcal enterotoxin B: stimulation of mature T cells and clonal deletion in neonatal mice. Cell 56: 27-35, 1989. PubMed: 2521300

Itohara S, et al. Monoclonal antibodies specific to native murine T-cell receptor gamma delta: analysis of gamma delta T cells during thymic ontogeny and in peripheral lymphoid organs. Proc. Natl. Acad. Sci. USA 86: 5094-5098, 1989. PubMed: 2787028

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Kubo RT, et al. Characterization of a monoclonal antibody which detects all murine alphabeta T cell receptors. J. Immunol. 142: 2736-2742, 1989. PubMed: 2467936

White J, et al. The V beta-specific superantigen staphylococcal enterotoxin B: stimulation of mature T cells and clonal deletion in neonatal mice. Cell 56: 27-35, 1989. PubMed: 2521300

Itohara S, et al. Monoclonal antibodies specific to native murine T-cell receptor gamma delta: analysis of gamma delta T cells during thymic ontogeny and in peripheral lymphoid organs. Proc. Natl. Acad. Sci. USA 86: 5094-5098, 1989. PubMed: 2787028