H57-597 (ATCC® HB-218)

Organism: Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

View Permits

Organism Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with all alpha,beta TCR bearing murine T cells, but does not react with receptors on gamma,delta positive T cells.
The antibody can directly activate alpha,beta TCR bearing T cells and can be used to immunoprecipitate the receptor from T cell lysates.
Derivation
Animals were immunized with affinity-purified T cell antigen receptor (TCR) from the murine T cell hybridoma DO-11.10.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse T cell antigen receptor
Cellular Products
immunoglobulin; monoclonal antibody; against mouse T cell antigen receptor
Comments
Animals were immunized with affinity-purified T cell antigen receptor (TCR) from the murine T cell hybridoma DO-11.10.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with all alpha,beta TCR bearing murine T cells, but does not react with receptors on gamma,delta positive T cells.
The antibody can directly activate alpha,beta TCR bearing T cells and can be used to immunoprecipitate the receptor from T cell lysates.
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere
  • 0.05 mM 2-Mercaptoethanol

Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG
Name of Depositor RT Kubo
References

Kubo RT, et al. Characterization of a monoclonal antibody which detects all murine alphabeta T cell receptors. J. Immunol. 142: 2736-2742, 1989. PubMed: 2467936

White J, et al. The V beta-specific superantigen staphylococcal enterotoxin B: stimulation of mature T cells and clonal deletion in neonatal mice. Cell 56: 27-35, 1989. PubMed: 2521300

Itohara S, et al. Monoclonal antibodies specific to native murine T-cell receptor gamma delta: analysis of gamma delta T cells during thymic ontogeny and in peripheral lymphoid organs. Proc. Natl. Acad. Sci. USA 86: 5094-5098, 1989. PubMed: 2787028

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Kubo RT, et al. Characterization of a monoclonal antibody which detects all murine alphabeta T cell receptors. J. Immunol. 142: 2736-2742, 1989. PubMed: 2467936

White J, et al. The V beta-specific superantigen staphylococcal enterotoxin B: stimulation of mature T cells and clonal deletion in neonatal mice. Cell 56: 27-35, 1989. PubMed: 2521300

Itohara S, et al. Monoclonal antibodies specific to native murine T-cell receptor gamma delta: analysis of gamma delta T cells during thymic ontogeny and in peripheral lymphoid organs. Proc. Natl. Acad. Sci. USA 86: 5094-5098, 1989. PubMed: 2787028