CDR2 (ATCC® HB-214)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The primary specificity is for the thymic cortex, although there is faint non-epithelial reactivity in the medulla.
It cross-reacts with epithelial elements in human kidney, tonsils, pancreatic ducts, lymph nodes and esophagus.
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human cortical thymic epithelium
Cellular Products
immunoglobulin; monoclonal antibody; against human cortical thymic epithelium
Comments
Animals were immunized with homogenized human thymus (1.5 to 2 years of age).
Spleen cells were fused with Sp2/0 myeloma cells.
The primary specificity is for the thymic cortex, although there is faint non-epithelial reactivity in the medulla.
The antibody does not react with mouse or rat tissues.
It cross-reacts with epithelial elements in human kidney, tonsils, pancreatic ducts, lymph nodes and esophagus.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Complete Growth Medium
Culture Medium: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum,
10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG2b
Name of Depositor RV Rouse
References

Rouse RV, et al. Monoclonal antibodies reactive with subsets of mouse and human thymic epithelial cells. J. Histochem. Cytochem. 36: 1511-1517, 1988. PubMed: 2461413

Basic Documentation
References

Rouse RV, et al. Monoclonal antibodies reactive with subsets of mouse and human thymic epithelial cells. J. Histochem. Cytochem. 36: 1511-1517, 1988. PubMed: 2461413