1G1 [LS141-1G1-65-15-1] (ATCC® HB-12624)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma:lymphoblast B lymphocyte; somatic cell hybri  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma:lymphoblast B lymphocyte; somatic cell hybri
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:  C57BL6 (B cell); BALB/c (myeloma)

Applications
The cells produce immunoglobulin; monoclonal antibody; against human Chemokine Receptor 4 (CCR4).
Storage Conditions liquid nitrogen vapor phase
Images
Derivation
Animals were immunized with L1.2 cells expressing high levels of transfected CCR4. Spleen cells were fused with Sp2/0 myeloma cells. The cells produce immunoglobulin; monoclonal antibody; against human Chemokine Receptor 4 (CCR4).
Comments
Animals were immunized with L1.2 cells expressing high levels of transfected CCR4. Spleen cells were fused with Sp2/0 myeloma cells. The cells produce immunoglobulin; monoclonal antibody; against human Chemokine Receptor 4 (CCR4).
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10(4) viable cells/ml. Maintain cell density between 4 X 10(4) and 4 X 10(5) viable cells/ml. Do not exceed 7 X10(5) cells/ ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium, extra 10% FBS; 7.5% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Isotype IgG1 kappa
Population Doubling Time 19 hours
Name of Depositor LeukoSite, Inc.
U.S. Patent Number
References

Wu L, et al. Anti-CCR4 antibodies and methods of use therefor. US Patent 6,488,930 dated Dec 3 2002

Wilbanks A, et al. Expression cloning of the STRL33/BONZO/TYMSTRligand reveals elements of CC, CXC, and CX3C chemokines. J. Immunol. 188: 5145-5154, 2001. PubMed: 11290797

Jones D, et al. Expression pattern of T-cell-associated chemokine receptors and their chemokines correlates with specific subtypes of T-cell non-Hodgkin lymphoma. Blood 96: 685-690, 2000. PubMed: 10887135

Kim CH, et al. Rules of chemokine receptor association with T cell polarization in vivo. J. Clin. Invest. 108: 1331-1339, 2001. PubMed: 11696578

Campbell JJ, et al. The chemokine receptor CCR4 in vascular recognition by cutaneous but not intestinal memory T cells. Nature 400: 776-780, 1999. PubMed: 10466728

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Wu L, et al. Anti-CCR4 antibodies and methods of use therefor. US Patent 6,488,930 dated Dec 3 2002

Wilbanks A, et al. Expression cloning of the STRL33/BONZO/TYMSTRligand reveals elements of CC, CXC, and CX3C chemokines. J. Immunol. 188: 5145-5154, 2001. PubMed: 11290797

Jones D, et al. Expression pattern of T-cell-associated chemokine receptors and their chemokines correlates with specific subtypes of T-cell non-Hodgkin lymphoma. Blood 96: 685-690, 2000. PubMed: 10887135

Kim CH, et al. Rules of chemokine receptor association with T cell polarization in vivo. J. Clin. Invest. 108: 1331-1339, 2001. PubMed: 11696578

Campbell JJ, et al. The chemokine receptor CCR4 in vascular recognition by cutaneous but not intestinal memory T cells. Nature 400: 776-780, 1999. PubMed: 10466728