1G1 [LS141-1G1-65-15-1] (ATCC® HB-12624)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma:lymphoblast B lymphocyte; somatic cell hybri  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma:lymphoblast B lymphocyte; somatic cell hybri
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Strain

Strain:  C57BL6 (B cell); BALB/c (myeloma)

Applications
The cells produce immunoglobulin; monoclonal antibody; against human Chemokine Receptor 4 (CCR4).
Storage Conditions liquid nitrogen vapor phase
Images
Derivation
Animals were immunized with L1.2 cells expressing high levels of transfected CCR4. Spleen cells were fused with Sp2/0 myeloma cells. The cells produce immunoglobulin; monoclonal antibody; against human Chemokine Receptor 4 (CCR4).
Comments
Animals were immunized with L1.2 cells expressing high levels of transfected CCR4. Spleen cells were fused with Sp2/0 myeloma cells. The cells produce immunoglobulin; monoclonal antibody; against human Chemokine Receptor 4 (CCR4).
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10(4) viable cells/ml. Maintain cell density between 4 X 10(4) and 4 X 10(5) viable cells/ml. Do not exceed 7 X10(5) cells/ ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium, extra 10% FBS; 7.5% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Isotype IgG1 kappa
Population Doubling Time 19 hours
Name of Depositor LeukoSite, Inc.
References

Wu L, et al. Anti-CCR4 antibodies and methods of use therefor. US Patent 6,488,930 dated Dec 3 2002

Wilbanks A, et al. Expression cloning of the STRL33/BONZO/TYMSTRligand reveals elements of CC, CXC, and CX3C chemokines. J. Immunol. 188: 5145-5154, 2001. PubMed: 11290797

Jones D, et al. Expression pattern of T-cell-associated chemokine receptors and their chemokines correlates with specific subtypes of T-cell non-Hodgkin lymphoma. Blood 96: 685-690, 2000. PubMed: 10887135

Kim CH, et al. Rules of chemokine receptor association with T cell polarization in vivo. J. Clin. Invest. 108: 1331-1339, 2001. PubMed: 11696578

Campbell JJ, et al. The chemokine receptor CCR4 in vascular recognition by cutaneous but not intestinal memory T cells. Nature 400: 776-780, 1999. PubMed: 10466728

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Wu L, et al. Anti-CCR4 antibodies and methods of use therefor. US Patent 6,488,930 dated Dec 3 2002

Wilbanks A, et al. Expression cloning of the STRL33/BONZO/TYMSTRligand reveals elements of CC, CXC, and CX3C chemokines. J. Immunol. 188: 5145-5154, 2001. PubMed: 11290797

Jones D, et al. Expression pattern of T-cell-associated chemokine receptors and their chemokines correlates with specific subtypes of T-cell non-Hodgkin lymphoma. Blood 96: 685-690, 2000. PubMed: 10887135

Kim CH, et al. Rules of chemokine receptor association with T cell polarization in vivo. J. Clin. Invest. 108: 1331-1339, 2001. PubMed: 11696578

Campbell JJ, et al. The chemokine receptor CCR4 in vascular recognition by cutaneous but not intestinal memory T cells. Nature 400: 776-780, 1999. PubMed: 10466728