558-D human x mouse (heterohybridoma) (HB-10894)

Designation / Description 558-D human x mouse (heterohybridoma)
U.S. Patent Number
Biosafety Level 2 Cells contain Human Immunodeficiency virus type 1 (HIV-1)

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Human peripheral blood mononuclear cells from HIV seropositive patients were transformed with Epstein-Barr virus (EBV).
Antibody producing clones were expanded and fused with SHM-D33 heterohybridoma cells (ATCC CRL-1668).
The antibody is specific for the CD4-binding domain of the gp120 protein of HIV-1; it inhibits CD4-rgp120 binding. It has broad HIV group specificity and reacts with the HTLV-IIIB, MN and SF-2 strains of HIV-1.
The antibody can be used in ELISA and neutralization assays.
Shipped frozen
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Zolla-Pazner S, et al. Human monoclonal antibodies to the CD4-binding domain of HIV, uses thereof and synergistic neutralization of HIV. US Patent 6,241,986 dated Jun 5 2001

Wang H, et al. Human monoclonal and polyclonal anti-human immunodeficiency virus-1 antibodies share a common clonotypic specificity. Eur. J. Immunol. 22: 1749-1755, 1992. PubMed: 1378015