psi 2 BAG alpha ATCC ® CRL-9560™ Mus musculus

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism	Mus musculus, mouse
Cell Type	fibroblast
Product Format	frozen
Culture Properties	adherent
Biosafety Level	1
Age	embryo
Strain	NIH/Swiss
Applications	produces protein beta-galactosidase beta-D-galactosidase

Derivation	The psi 2 BAG alpha cell line has produced an infectious helper virus upon prolonged passage, but present stocks do not. The cell line was derived from the psi 2 cell line developed by Mann, et al. (Cell 33:153-159, 1983) from the NIH/3T3 cell line (ATCC CRL-1658).
Genes Expressed	a beta galactosidase transducing vector (BAG),beta-galactosidase(beta-D-galactosidase) [lacZ],beta-galactosidase
Comments	The psi 2 BAG alpha cell line has produced an infectious helper virus upon prolonged passage, but present stocks do not. The BAG construct has been used to transfect psiCRE cells in ATCC CRL 1858 and has been used to transfect psi2 cells in ATCC CRL 9560. This line produces a vector (BAG) that can infect and mouse and rat and transduce the bacterial beta galactosidase gene. The BAG vector has been used to mark cells for lineage analysis based on the histochemical detection of the bacterial beta galactosidase. The cell line was derived from the psi 2 cell line developed by Mann, et al. (Cell 33:153-159, 1983) from the NIH/3T3 cell line (ATCC CRL-1658). This line may produce an infectious helper virus upon prolonged passage, present stocks do not. A protocol to test for the helper virus is available and accompanies the cells when ordered. The vector has been used to infect a variety of neural cell types and to express beta-galactosidase and resistance to G418.

Complete Growth Medium	Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; bovine calf serum, 10%
Subculturing	Medium Renewal: Every 2 to 3 days Remove medium, add fresh 0.25% Trypsin - 0.02% EDTA and allow the flask to sit at room temperature (or 37C) until the cells detach (2 to 3 minutes). Add fresh medium, aspirate and dispense into new flasks.
Culture Conditions	Temperature: 37.0°C

Name of Depositor	Harvard Medical School
Passage History	The psi 2 BAG alpha cell line has produced an infectious helper virus upon prolonged passage, but present stocks do not. This line may produce an infectious helper virus upon prolonged passage, present stocks do not.
Year of Origin	1983
References	Price J, et al. Lineage analysis in the vertebrate nervous system by retrovirus-mediated gene transfer. Proc. Natl. Acad. Sci. USA 84: 156-160, 1987. PubMed: 3099292 Turner DL, Cepko CL. A common progenitor for neurons and glia persists in rat retina late in development. Nature 328: 131-136, 1987. PubMed: 3600789 Mann R, et al. Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus. Cell 33: 153-159, 1983. PubMed: 6678608

Permits

These permits may be required for shipping this product:

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

Basic Documentation

Product Sheet

Certificate of Analysis

MSDS

References

Price J, et al. Lineage analysis in the vertebrate nervous system by retrovirus-mediated gene transfer. Proc. Natl. Acad. Sci. USA 84: 156-160, 1987. PubMed: 3099292

Turner DL, Cepko CL. A common progenitor for neurons and glia persists in rat retina late in development. Nature 328: 131-136, 1987. PubMed: 3600789

Mann R, et al. Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus. Cell 33: 153-159, 1983. PubMed: 6678608

psi 2 BAG alpha (ATCC® CRL-9560™)

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psi 2 BAG alpha (ATCC^® CRL-9560^™)