H9/HTLV-IIIB (ATCC® CRL-8543)

Organism: Homo sapiens, human  /  Cell Type: T lymphocyte  / 

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Organism Homo sapiens, human
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells Contain RETROVIRUS
Applications
The cells produce HTLV III.
Genes Expressed
Human T cell leukemia virus III (HTLV III); Human Immunodeficiency virus (HIV)
Cellular Products
Human T cell leukemia virus III (HTLV III); Human Immunodeficiency virus (HIV)
Comments
The cells produce HTLV III.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Culture Conditions
Temperature: 37.0°C
Name of Depositor The United States of America
References

Popovic M, et al. Serological detection of antibodies to HTLV-III in sera of patients with AIDS and pre-AIDS conditions. US Patent 4,520,113 dated May 28 1985

Chang SY, et al. The origin of HIV-1 isolate HTLV-IIIB. Nature 363: 466-469, 1993. PubMed: 8502298

Trkola A, et al. Human momoclonal antibody 2G12 defines a distinctive neutralization epitope on the gp120 glycoprotein of human immunodeficiency virus type 1. J. Virol. 70: 1100-1108, 1996. PubMed: 8551569

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Popovic M, et al. Serological detection of antibodies to HTLV-III in sera of patients with AIDS and pre-AIDS conditions. US Patent 4,520,113 dated May 28 1985

Chang SY, et al. The origin of HIV-1 isolate HTLV-IIIB. Nature 363: 466-469, 1993. PubMed: 8502298

Trkola A, et al. Human momoclonal antibody 2G12 defines a distinctive neutralization epitope on the gp120 glycoprotein of human immunodeficiency virus type 1. J. Virol. 70: 1100-1108, 1996. PubMed: 8551569